Whey protein isolate: a versatile dairy-derived hydrogel for bone and vascular tissue engineering and antimicrobial applications Abstract
Bočna strana članka
Glavni sadržaj članka
Apstrakt
Whey Protein Isolate (WPI) is an inexpensive by-product of the dairy industry, available in large quantities and used as a dietary supplement. WPI is over 97% protein; three-quarters is beta-lactoglobulin (β-LG). WPI in cell culture medium promoted the proliferation and differentiation of bone-forming cells. Solutions of WPI can be heated to form hydrogels, which withstand sterilization by autoclaving; an important practical advantage. Denaturation of β-LG leads to increased hydrophobic interactions and disulphide bond formation and thus crosslinking to form the polymer hydrogel network. We have studied WPI hydrogels as scaffolds for bone-forming cells and carriers of hydrophobic substances.
Detalji članka
Rubrika
Ovaj rad je pod Creative Commons Aуторство-Nekomercijalno-Bez prerade 4.0 Internacionalna licenca.
Kada je rukopis prihvaćen za objavlјivanje, autori prenose autorska prava na izdavača. U slučaju da rukopis ne bude prihvaćen za štampu u časopisu, autori zadržavaju sva prava.
Na izdavača se prenose sledeća prava na rukopis, uklјučujući i dodatne materijale, i sve delove, izvode ili elemente rukopisa:
- pravo da reprodukuje i distribuira rukopis u štampanom obliku, uklјučujući i štampanje na zahtev;
- pravo na štampanje probnih primeraka, reprint i specijalnih izdanja rukopisa;
- pravo da rukopis prevede na druge jezike;
- pravo da rukopis reprodukuje koristeći fotomehanička ili slična sredstva, uklјučujući, ali ne ograničavajući se na fotokopiranje, i pravo da distribuira ove kopije;
- pravo da rukopis reprodukuje i distribuira elektronski ili optički koristeći sve nosioce podataka ili medija za pohranjivanje, a naročito u mašinski čitlјivoj/digitalizovanoj formi na nosačima podataka kao što su hard disk, CD-ROM, DVD, Blu-ray Disc (BD), mini disk, trake sa podacima, i pravo da reprodukuje i distribuira rukopis sa tih prenosnika podataka;
- pravo da sačuva rukopis u bazama podataka, uklјučujući i onlajn baze podataka, kao i pravo prenosa rukopisa u svim tehničkim sistemima i režimima;
- pravo da rukopis učini dostupnim javnosti ili zatvorenim grupama korisnika na osnovu pojedinačnih zahteva za upotrebu na monitoru ili drugim čitačima (uklјučujući i čitače elektonskih knjiga), i u štampanoj formi za korisnike, bilo putem interneta, onlajn servisa, ili putem internih ili eksternih mreža.
Kako citirati
Reference
Keppler J. K. et al., Differences in heat stability and ligand binding among β-lactoglobulin genetic variants A, B and C using (1)H NMR and fluorescence quenching Biochim Biophys Acta 2014;1844(6):1083-93
Douglas, T.E.L et a., Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells J Dairy Sci. 2018; 101(1):28-36
Orlien V., Utilizing High Pressure Processing to Induce Structural Changes in Dairy and Meat Products, Reference Module in Food Science 2016, First Edition, 1–9
Norris, K. et al. Marine-inspired enzymatic mineralization of dairy-derived whey protein isolate (WPI) hydrogels for bone tissue regeneration Marine Drugs 2020; 18, 6-18.
Genç, H. et al. Endothelialization of Whey Protein Isolate-Based Scaffolds for Tissue Regeneration, Molecules 2023, 28(20), 7052
Dziadek, M. et al. Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering, Mater Des 2021; 205, 109749
Dziadek, M. et al. Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering, J Biomed Mater Res A 2019; 107, 2479-2491.
Gupta, D. et al. Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration, Mater Des 2020; 188, 108408
Klimek K. et al. Could Curdlan/Whey Protein Isolate/Hydroxyapatite Biomaterials Be Considered as Promising Bone Scaffolds?—Fabrication, Characterization, and Evaluation of Cytocompatibility towards Osteoblast Cells In Vitro, Cells 2022; 11(20), 3251
Platania V. et al. Phloroglucinol-enhanced whey protein isolate hydrogels with antimicrobial activity for tissue engineering, Mater Sci Eng C 2021; 129, 112412
Baines, D., et al. The enrichment of whey protein isolate hydrogels with Poly-γ-glutamic acid promotes the proliferation and osteogenic differentiation of pre-osteoblasts, Gels, accepted 19/12/2023