Enhanced fertilization effect of a compost obtained from mixed herbs waste inoculated with novel strains of mesophilic bacteria

Snežana M. Dimitrijević, Dragoja S. Radanović, Svetlana B. Antić-Mladenović, Milica D. Milutinović, Mirjana D. Rajilić-Stojanović, Suzana I. Dimitrijević-Branković


Mixed medicinal plant waste was composted with addition of novel bacterial strains belonging to the genera Streptomyces, Paenybacillus, Bacillus and Hymenobacter. The composting was followed by assessment of chemical and biological parameters including C/N ratio, loss of organic matter, phosphorous and potassium content as well as CO2 generation and dehydrogenase activity during 164 days. The selected mesophilic bacterial starters had a potential to significantly reduce the period of mixed herb waste decomposition, from about 6 months to about 2.5 months. Based on the seed germination index of four plants (Fagopirum esculentum, Thymus vulgaris, Cynara scolimus and Lavandula officinalis) the germination and radial root growth of the investigated plants was improved by the inoculated compost. The germination index of all tested species on the mature inoculated composts was in average 60% higher compared to the control compost. The research indicates that the mesophilic starter addition into the herbs waste can contribute to the speed of waste decomposition and lead to the improvement of biofertilization effect of the obtained compost.


composting; herbs waste; mesophilic bacteria; germination index; plant growth stimulation; phytotoxicity

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Gaind S, Nain L, Patel VB. Quality evaluation of co-composted wheat straw, poultry droppings and oil seed cakes. Biodegradation. 2009; 20: 307-317.

Mehta CM, Palni U, Franke-Whittle IH, Sharma AK. Compost: Its role, mechanism and impact on reducing soil-borne plant diseases. Waste Management. 2014; 34: 607-622.

Boulter JI, Boland GJ, Trevors JT. Compost: A study of the development process and end-product potential for suppression of turfgrass disease. World J Microbiol Biotechnol. 2000; 16: 115-134.

Wu L, Ma LQ, Martinez GA. Comparison of Methods for Evaluating Stability and Maturity of Biosolids Compost. J Environ Qual. 2000; 29: 424-429.

Hue NV, Liu J. Predicting Compost Stability. Compost Sci Util. 1995; 3: 8-15.

Qian X, Shen G, Wang Z, Guo C, Liu Y, Lei Z, Zhang Z. Co-composting of livestock manure with rice straw: Characterization and establishment of maturity evaluation system. Waste Management. 2014; 34: 530-535.

Rashad FM, Saleh WD, Moselhy MA. Bioconversion of rice straw and certain agro-industrial wastes to amendments for organic farming systems: 1. Composting, quality, stability and maturity indices. Bioresour Technol. 2010; 101: 5952-5960.

Kasana R, Salwan R, Dhar H, Dutt S, Gulati A. A Rapid and Easy Method for the Detection of Microbial Cellulases on Agar Plates Using Gram’s Iodine. Curr Microbiol. 2008; 57: 503-507.

Hossain N, Rahman M. Antagonistic activity of antibiotic producing Streptomyces sp. against fish and human pathogenic bacteria. Braz Arch Biol Technol. 2014; 57: 233-237

Rahman MA, Islam MZ, Islam MAU. Antibacterial Activities of Actinomycete Isolates Collected from Soils of Rajshahi, Bangladesh. Biotechnol Res Int. 2011; 2011: 6.

Tiquia SM, Tam NFY. Composting of spent pig litter in turned and forced-aerated piles. Environ Pollut. 1998; 99: 329-337.

Bremner JM, Keeney DR. Steam distillation methods for determination of ammonium, nitrate and nitrite, Anala Chim Acta. 1965; 32: 485-495.

AOAC Official methods of analysis: association of official analytical chemist, EUA. In; 2000.

Faithfull NT. Methods in Agricultural Chemical Analysis: A Practical Handbook. CABI Publishing, Cambridge, MA, USA; 2003.

Albalasmeh AA, Berhe AA, Ghezzehei TA. A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry, Carbohyd Polym. 2013; 97: 253-261.

Iannotti DA, Pang T, Toth BL, Elwell DL, Keener HM, Hoitink HAJ. A Quantitative Respirometric Method for Monitoring Compost Stability. Compost Sci Util. 1993; 1: 52-65.

Tiquia SM, Tam NFY, Hodgkiss IJ. Effects of composting on phytotoxicity of spent pig-manure sawdust litter. Environmental Pollution. 1996; 93: 249-256.

Warman PR. Evaluation of Seed Germination and Growth Tests for Assessing Compost Maturity. Compost Sci Util. 1999; 7: 33

Lepš J, Šmilauer P. Multivariate analysis of ecological data using CANOCO. Cambridge University Press, Cambridge; 2003.

Scalbert A. Antimicrobial properties of tannins. Phytochemistry. 1991; 30: 3875-3883.

Siqueira CFdQ, Cabral DLV, Peixoto Sobrinho TJdS, de Amorim ELC, de Melo JG, Araújo TAdS, de Albuquerque UP. Levels of Tannins and Flavonoids in Medicinal Plants: Evaluating Bioprospecting Strategies. Evid Based Complement Alternat Med. 2012; 2012: 7.

Chalkos D, Kadoglidou K, Karamanoli K, Fotiou C, Pavlatou-Ve A, Eleftherohorinos I, Constantinidou H-I, Vokou D. Mentha spicata and Salvia fruticosa composts as soil amendments in tomato cultivation. Plant Soil. 2010; 332: 495-509.

Tiquia SM, Tam NFY. Characterization and composting of poultry litter in forced-aeration piles. Process Biochem. 2002; 37: 869-880.

Paradelo R, Moldes AB, Barral MT. Evolution of organic matter during the mesophilic composting of lignocellulosic winery wastes. J Environ Manage. 2013; 116: 18-26.

Khan M, Ueno K, Horimoto S, Komai F, Tanaka K, Ono Y. Physicochemical, including spectroscopic, and biological analyses during composting of green tea waste and rice bran. Biol Fertil Soils. 2009; 45: 305-313.

Said-Pullicino D, Erriquens FG, Gigliotti G. Changes in the chemical characteristics of water-extractable organic matter during composting and their influence on compost stability and maturity. Bioresource Technology. 2007; 98: 1822-1831.

Mihajlovski K, Carević M, Dević M, Šiler-Marinković S, Rajilić-Stojanović M, Dimitrijević-Branković S. Lignocellulosic waste material as substrate for Avicelase production by a new strain of Paenibacillus chitinolyticus CKS1. Int Biodeterior Biodegradation. 2015; 104: 426-434.

Hubbe MA, Nazhad M, Sánchez C. Composting as a way to convert cellulosic biomass and organic waste into high-value soil amendments: a review. Bioresources. 2010; 5: 2808-2854

Franke-Whittle IH, Confalonieri A, Insam H, Schlegelmilch M, Körner I. Changes in the microbial communities during co-composting of digestates. Waste Management. 2014; 34: 632-641.

Moldes A, Vázquez M, Domínguez J, Díaz-Fierros F, Barral M. Evaluation of mesophilic biodegraded grape marc as soil fertilizer. Appl Biochem Biotechnol. 2007; 141: 27-36.

Kumar V, Singh KP. Enriching vermicompost by nitrogen fixing and phosphate solubilizing bacteria. Bioresource Technology. 2001; 76: 173-175.

Meunchang S, Panichsakpatana S, Weaver R. Inoculation of sugar mill by-products compost with N2-fixing bacteria. Plant Soil. 2005; 271: 219-225.

Nair J, Okamitsu K. Microbial inoculants for small scale composting of putrescible kitchen wastes. Waste Management. 2010; 30: 977-982.

Forster J, Zech W, Würdinger E. Comparison of chemical and microbiological methods for the characterization of the maturity of composts from contrasting sources. Biol Fertil Soils. 1993; 16: 93-99.

Benito M, Masaguer A, Moliner A, Arrigo N, Palma R. Chemical and microbiological parameters for the characterisation of the stability and maturity of pruning waste compost. Biol Fertil Soils. 2003; 37: 184-189.

Komilis DP, Tziouvaras IS. A statistical analysis to assess the maturity and stability of six composts. Waste Management. 2009; 29: 1504-1513.

López-González JA, Suárez-Estrella F, Vargas-García MC, López MJ, Jurado MM, Moreno J. Dynamics of bacterial microbiota during lignocellulosic waste composting: Studies upon its structure, functionality and biodiversity. Bioresour Technol. 2015; 175: 406-416.

DOI: https://doi.org/10.2298/HEMIND170327013D

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