Optimization of spray drying conditions for production of Achillea millefolium extract powder Original scientific paper

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Anđela N. Radisavljević
Milica Milutinović
Suzana Dimitrijević Branković
Petar Uskoković
Mirjana Rajilić-Stojanović


In this study, a spray drying process of yarrow (Achillea millefolium L.) liquid extracts was optimized by using the response surface methodology. The study aimed to determine the influence of temperature (120-195 °C), liquid flow rate (3-16.5 cm3 min-1) and dry matter content in the liquid extract (0.3-1.5 %) on the yield of the drying process, the total polyphenols content and the antioxidant activity of the dry powder. Under the tested conditions the yield varied dramatically and ranged from 8 to 75 %, while the effects on the polyphenol content and antioxidant activity were lower. The optimized conditions for the maximum antioxidant activity and maximal yield of the dried extract were as follows: temperature of 130 °C, liquid flow rate of 7.5 cm3 min-1 and dry matter content of 1.2 %. Under the optimal conditions, the yield was 66 %, while there was a slight decrease in the polyphenol content in the dried extract as compared to that in the liquid extract (145 mg of gallic acid equivalents [GAE] per g of the total dry matter vs. 152 mg GAE g-1, respectively). Consequently, antioxidant activity of the dry powder was only slightly reduced as compared to that of the liquid extract (DPPH neutralization was 58 vs. 64 %, respectively). The dried yarrow powder preserved its antimicrobial activity against pathogenic bacteria Staphylococcus aureus (MIC value of 10 mg cm-3) and Pseudo­monas aeruginosa (MIC value of 20 mg cm-3).


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Radisavljević, A. N., Milutinović, M. ., Dimitrijević Branković, S. ., Uskoković, P. ., & Rajilić-Stojanović, M. . . (2022). Optimization of spray drying conditions for production of Achillea millefolium extract powder: Original scientific paper. HEMIJSKA INDUSTRIJA, 75(6), 353–363. https://doi.org/10.2298/HEMIND210527030R
Biochemical Engineering - General


Palhares RM, Gonçalves Drummond M, dos Santos Alves Figueiredo Brasil B, Pereira Cosenza G, das Graças Lins Brandao M, Oliveira G. Medicinal plants recommended by the world health organization: DNA barcode identification associated with chemical analyses guarantees their quality. PloS one. 2015;10:e0127866. https://doi.org/10.1371/journal.pone.0127866

Popovic V, Tatic M, Sikora V, Ikanovic J, Drazic G, Djukic V, Mihailovic B, Filipovic V, Dozet G, Jovanovic Lj, Stevanovic P. Variability of yield and chemical composition in soybean genotypes grown under different agro-ecological conditions of Serbia. Rom Agric Res. 2016;33:29-39.

Pham HNT, Nguyen VT, Vuong QV, Bowyer MC, Scarlett CJ. Effect of extraction solvents and drying methods on the physicochemical and antioxidant properties of Helicteres hirsuta Lour. leaves. Technol. 2015;3:285-301. https://doi.org/10.3390/technologies3040285

Azmin SNHM, Manan ZA, Alwi SRW, Chua LS, Mustaffa AA, Yunus NA. Herbal processing and extraction technologies. Sep Purif Rev. 2016;45:305-320. https://doi.org/10.1080/15422119.2016.1145395

Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products:a comprehensive review. Chin Med-UK. 2018;13:2-26. https://doi.org/10.1186/s13020-018-0177-x

Lakshmi T, Geetha RV, Roy A, Kumar SA. Yarrow (Achillea Millefolium Linn.) a herbal medicinal plant with broad therapeutic use-A review. Int J Pharm Sci Rev Res. 2011;9:136-141.

Cavalcanti AM, Baggio CH, Freitas CS, Rieck L, de Sousa RS, Da Silva-Santos JE, Mesia-Vela S, Marques MC. Safety and antiulcer efficacy studies of Achillea millefolium L. after chronic treatment in Wistar rats. J Ethnopharmacol. 2006;107:277-284. https://doi.org/10.1016/j.jep.2006.03.011

Benedek B, Kopp B. Achillea millefolium L. s.l. revisited:Recent findings confirm the traditional use. Wien Med Wochenschr. 2007;157:312-314. https://doi.org/10.1007/s10354-007-0431-9

Saeidnia S, Gohari AR, Mokhber-Dezfuli N, Kiuchi F. A review on phytochemistry and medicinal properties of the genus Achillea. DARU. 2011;19:173-86.

Othman L, Sleiman A, Abdel-Massih RM. Antimicrobial activity of polyphenols and alkaloids in Middle Eastern plants. Front Microbiol. 2019;10:911. https://doi.org/10.3389/fmicb.2019.00911

Milutinovic M, Radovanovic N, Corovic M, Siler-Marinkovic S, Rajilic-Stojanovic M, Dimitrijevic-Brankovic S. Optimisation of microwave-assisted extraction parameters for antioxidants from waste Achillea millefolium dust. Ind Crop Prod. 2015;77:333-341. https://doi.org/ 10.1016/j.indcrop.2015.09.007

Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr J TraditComplem. 2011;8:1-10. https://doi.org/10.4314/ajtcam.v8i1.60483

Oliveira WP, Bott RB, Souza CRF. Manufacture of standardized dried extracts from medicinal Brazilian plants. Dry Technol. 2006;24:523-533. https://doi.org/10.1080/07373930600612073

Bakowska-Barczak AM, Kolodziejczyk PP. Black currant polyphenols:their storage stability and microencapsulation. Ind Crop Prod. 2011;34:1301-1309. https://doi.org/10.1016/j.indcrop.2010.10.002

Rocha GA, Favaro-Trindade CS, Grosso CRF. Microencapsulation of lycopene by spray drying:Characterization, stability and application of microcapsules. Food Bioprod Process. 2012;90:1-6. https://doi.org/10.1016/j.fbp.2011.01.001

Yatsu FKJ, Borghetti GS, Bassani VL. Technological characterization and stability of Ilex paraguariensis St. Hil. Aquifoliaceae (Mate) spray-dried powder. J Med Food. 2011;14:413-419. https://doi.org/10.1089/jmf.2010.0044

Souza CRF, Oliveira WP. Powder properties and system behaviour during spray drying of Bauhinia forficata link extract. Dry Technol. 2006;24:735-749. https://doi.org/10.1080/07373930600685905

Cortes-Rojas DF, Oliveira WP. Physicochemical properties of phytopharmaceutical preparations as affected by drying methods and carriers. Dry Technol. 2012;30:921-934. https://doi.org/10.1080/07373937.2012.666608

Amaro MI, Tajber L, Corrigan OI, Healy AM. Optimisation of spray drying process conditions for sugar nanoporousmicroparticles (NPMPs) intended for inhalation. Int J Pharm. 2011;421:99-109. https://doi.org/10.1016/j.ijpharm.2011.09.021

Nedovic V, Kalusevic A, Manojlovic V, Petrovic T, Bugarski B. Encapsulation Systems in the Food Industry. In:Advances in Food Process Engineering Research and Applications, Boston, MA:Springer Nature America, Inc;2013:229-253. https://doi.org/10.1007/978-1-4614-7906-2_13

Chegini GR, Ghobadian B. Spray dryer parameters for fruit juice drying. World J Agric Sci. 2007;3:230-236.

Patel RP, Patel MP, Suthar AM. Spray drying technology:an overview. Indian J Sci Technol. 2009;2:44-47. https://doi.org/10.17485/ijst/2009/v2i10.3

Andrea T, Marcela F, Lucia C, Esther F, Elena M, Simona M. Microencapsulation of lipase and savinase enzymes by spray drying using Arabic gum as wall material. JEAS. 2016;6:161-173. https://doi.org/10.1007/978-1-4614-7906-2_1310.4236/jeas.2016.64012

Ozmen L, Langrish TAG. An experimental investigation of the wall deposition of milk powder in a pilot-scale spray dryer. Dry Technol. 2003;21:1253-1272. https://doi.org/10.1081/DRT-120023179

Kudra T. Sticky region in drying:Definition and identification. Dry Technol. 2003;21:1457-1469. https://doi.org/10.1081/DRT-120024678

Montgomery DC. Response Surface Methods and Designs. In:Design and analysis of experiments, Eighth Edition John Wiley and Sons, Inc, New Jersey;2005:478.

Singleton V, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965;16:144-158.

Lee SK, Mbwambo Z, Chung H, Luyengi L, Gamez E, Mehta R, Kinghorn A, Pezzuto J. Evaluation of the antioxidant potential of natural products. Comb Chem High Throughput Screen.1998;1:35.

Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007;42:321-324. https://doi.org/10.1016/j.ymeth.2007.01.006

Tontul I, Topuz A. Spray-drying of fruit and vegetable juices:Effect of drying conditions on the product yield and physical properties. Trends Food Sci Tech. 2017;63:91-102. https://doi.org/10.1016/j.tifs.2017.03.009

Billon A, Bataille B, Cassanas G, Jacob M. Development of spray-dried acetaminophen microparticles using experimental designs. Int J Pharm. 2000;203:159-168. https://doi.org/10.1016/s0378-5173(00)00448-8

Nguyen DQ, Nguyen TH, Mounir S, Allaf K. Effect of feed concentration and inlet air temperature on the properties of soymilk powder obtained by spray drying. Dry Technol. 2017;36(7):817-829. https://doi.org/10.1080/07373937.2017.1357040

Prinn KB, Costantino HR, Tracy M. Statistical modeling of protein spray drying at the lab scale. AAPS Pharm Sci Tech. 2002;3:E4. https://doi.org/10.1208/pt030104

Poozesh S, Jafari SM, Akafuah NK. Interrogation of a new inline multi-bin cyclone for sorting of produced powders of a lab-scale spray dryer. Powder Technol. 2020;373:590-598. https://doi.org/10.1016/j.powtec.2020.07.012

Maurya M, Murphyb K, Kumarb S, Shib L, Leea G. Effects of process variables on the powder yield of spray-dried trehalose on a laboratory spray-dryer. Eur J Pharm Biopharm. 2005;59:565-573. https://doi.org/10.1016/j.ejpb.2004.10.002

Gallo L, Llabot Juan M, Allemandi D, Bucala V, Pina J. Influence of spray-drying operating conditions on Rhamnus purshiana (Cascara sagrada) extract powder physical properties. Powder Technol. 2011;208:205-214. https://doi.org/10.1016/j.powtec.2010.12.021

Telang AM, Thorat BN. Optimization of process parameters for spray drying of fermented soy milk. Dry Technol. 2010;28:1445-1456. https://doi.org/10.1080/07373937.2010.482694

Toneli J, Park K, Negreiros A, Murr F. Spray-drying process optimization of chicory root inulin. Dry Technol. 2010;28:369-379. https://doi.org/10.1080/07373931003645017

Mihajlovic T, Ibric S, Mladenovic A. Application of design of experiments and multilayer perceptron neural network in optimization of the spray-drying process. Dry Technol. 2011;29:1638-1647. https://doi.org/10.1080/07373937.2011.592960

Tee LH, Chuah L, Pin KY, Abdull Rashih A, Yusof YA. Optimization of spray drying process parameters of Piper betleL. (Sirih) leaves extract coated with maltodextrin. J Chem Pharm Res. 2012;4:1833-1841.

Goula AM, Adamopoulos KG. Spray drying of tomato pulp in dehumidified air:I. The effect on product recovery. J Food Eng. 2005;66:25-34. https://doi.org/10.1016/j.jfoodeng.2004.02.029

Tonon RV, Brabet C, Hubinger MD. Influence of process conditions on the physicochemical properties of acai (Euterpe oleraceae Mart) powder produced by spray drying. J Food Eng. 2008;88:411-418. https://doi.org/10.1016/j.jfoodeng.2008.02.029

Ramos FdeM, Oliveira CCMde, Soares ASP, Silveira Junior V. Assessment of differences between products obtained in conventional and vacuum spray dryer. Food Sci Technol. 2016;36 (4):724-729. https://doi.org/10.1590/1678-457X.09216

Jafari SM, Ghalenoei MG, Dehnad D. Influence of spray drying on water solubility index, apparent density, and anthocyanin content of pomegranate juice powder. Powder Technol. 2017;311:59-65. https://doi.org/10.1016/j.powtec.2017.01.070

Pinon-Balderrama CI, Leyva-Porras C, Teran-Figueroa Y, Espinosa-Solis V, Alvarez-Salas C, Saavedra-Leos MZ. Encapsulation of Active Ingredients in Food Industry by Spray-Drying and Nano Spray-Drying Technologies. Processes. 2020;8 (8):889. https://doi.org/10.3390/pr8080889

Eldridge JA, Repko D, Mumper RJ. Retention of polyphenolic species in spray-dried blackberry extract using mannitol as a thermoprotectant. J Med Food. 2014;17:1064-1069. https://doi.org/10.1089/jmf.2013.0177

Bastias-Montes JM, Choque-Chavez MC, Alarcon-Enos J, Quevedo-Leon R, Munoz-Farina O, Vidal-San-Martin C. Effect of spray drying at 150, 160, and 170 °C on the physical and chemical properties of maqui extract (Aristotelia chilensis (Molina) Stuntz). Chil J Agric Res. 2019;79 (1):144-152. https://doi.org/10.4067/S0718-58392019000100144

Souza V, Thomazini M, Balieiro J, Trindade C. Effect of spray drying on the physicochemical properties and color stability of the powdered pigment obtained from vinification byproducts of the Bordo grape (Vitis labrusca). Food Bioprod Process. 2015;93:39-50. https://doi.org/10.1016/j.fbp.2013.11.001

Turkmen N, Sari F, Velioglu YS. The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chem. 2005;93:713-718. https://doi.org/10.1016/j.foodchem.2004.12.038

Sharma K, Ko EY, Assefa AD, Ha S, Nile SH, Lee ET, Park SW. Temperature-dependent studies on the total phenolics, flavonoids, antioxidant activities, and sugar content in six onion varieties. J Food Drug Anal. 2015;23:243-252. https://doi.org/10.1016/j.jfda.2014.10.005

Fang Z, Bhandari B. Effect of spray drying and storage on the stability of bayberry polyphenols. Food Chem. 2011;129:1139-1147. https://doi.org/10.1016/j.foodchem.2011.05.093

Edrisi M, Langrish TAG. Spray drying bioactive orange-peel extracts produced by Soxhlet extraction:Use of WPI, antioxidant activity and moisture sorption isotherms. LWT - Food Sci. Technol. 2016;72:1-8. https://doi.org/10.1016/j.lwt.2016.04.033

Frey FM. and Meyers R. Antibacterial activity of traditional medicinal plants used by Haudenosaunee peoples of New York State. BMC Complement Altern Med. 2010;10:64. https://doi.org/10.1186/1472-6882-10-64

Nostro A, Germano MP, D´Angelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol. 2000;30:379-384. https://doi.org/1046/j.1472-765x.2000.00731.x

Li Z-H, Cai M, Liu Y-S, Sun P-L, Luo S-L. Antibacterial activity and mechanisms of essential oil from Citrus medica L. var. sarcodactylis. Molecules. 2019;24:1577. https://doi.org/10.3390/molecules24081577

Tajik H, Jalali FSS, Sobhani A, Shahbazi Y, Zadeh MS. In vitro assessment of antimicrobial efficacy of alcoholic extract of Achillea millefolium in comparison with penicillin derivates. J Anim Vet Adv. 2008;7:508-511.

Lyss G, Glasl S, Jurenitsch J, Pahl HL, Merfort I. A sesquiterpene and sesquiterpene lactones from the Achillea millefolium group possess antiinflammatory properties but do not inhibit the transcription factor NF-γB. Pharm Pharmacol Lett. 2000;10:13-15.

Aljancic I, Vajs V, Menkovic N, Karadzic I, Juranic N, Milosavljevic S, Macura S. Flavones and sesquiterpene lactones from Achillea atrata subsp. multifida:antimicrobial activity. J Nat Prod. 1999;62:909-911. https://doi.org/10.1021/np980536m.

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