Aqueous extract of strawberry (Fragaria x ananassa Duch.) leaves as a stabilizing agent in the synthesis of bio-active silver nanoparticles
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The aim of the presented work was to investigate the potential of aqueous extract of cultivated strawberry (Fragaria x ananassa Duch.) leaves for stabilization of silver nanoparticles (AgNPs-E) synthesized at room (RT) and boiling temperature (BT). The synthesis and stability of AgNPs-E were monitored by UV-Vis spectroscopy confirming high stability of the AgNPs-E in the dark at room temperature. The Fourier-transform infrared spectra suggest that molecules containing oxygen and nitrogen functional groups (NH, (NH)C=O, CNO, C-O-C and OH) participate in the reduction and stabilization of formed nanoparticles. As determined by the DPPH test, AgNPs-E synthesized at RT exerted higher antioxidant activity as compared to AgNPs-E synthesized at BT (EC50 values of 0.025 and 0.039 mg cm-3, respectively). Also, the AgNPs-E synthesized at RT exerted higher antibacterial activity against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Bacillus subtilis and Bacillus luteus. Examination of the AgNPs-E on HeLa and MDCK cell lines showed concentration-dependent and cell line specific effects on the cell viability as evaluated by the MTT test. The obtained results indicate that synthesized AgNPs-E can be used as a base material in production of pharmaceutical preparations for potential skin applications.
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Ramsden J. Essentials of Nanotechnology, What is Nanotechnology, Ventus Publishing ApS; 2009.
Pramanik N, Bhattacharyya A, Kundu PP. Spectroscopic analysis and catalytic application of biopolymer capped silver nanoparticle, an effective antimicrobial agent. J Appl Polym Sci. 2015; 132(8): 41495.
Fatal T, Taştan P, Tüzün BS, Ozyazici M, Kivcak B. Synthesis characterization and studies on antioxidant activity of silver nanoparticles using Asphodelus aestivus Brot. aerial part extract. S Afr J Bot. 2017; 112: 346–353.
Cakić M, Glišić S, Cvetković D, Cvetinov M, Stanojević Lj, Danilović B, Cakić K. Green synthesis, characterization and antibacterial activity of silver nanoparticles produced from Fumaria officinalis L. plant extract. Colloid J. 2018; 80(6): 803–813.
Venkatadri B, Shanparvish E, Rameshkumar MR, Arasu MV, Al-Dhabi NA, Ponnusamy VK, Agastian P. Green synthesis of silver nanoparticles using aqueous rhizome extract of Zingiber officinale and Curcuma longa: In-vitro anti-cancer potential on human colon carcinoma HT-29 cells. Saudi J Biol Sci. 2020; 27: 2980–2986.
Das CGA, Kumar VG, Dhas TS, Karthick V, Govindaraju K, Joselin JM, Baalamurugan J. Antibacterial activity of silver nanoparticles (biosynthesis): A short review on recent advances. Biocatal Agric Biotechnol. 2020; 27: 101593.
MubarakAli D, Thajuddin N, Jeganathan K, Gunasekaran M. Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Colloids Surf B. 2011; 85(2): 360–365.
Amooaghaie R, Saeri MR, Azizi M. Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from Nigella sativa leaf extract in comparison with chemical silver nanoparticles. Ecotoxicol Environ Saf. 2015; 120: 400–408.
Lima RD, Seabra AB, Durán N. Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles. J Appl Toxicol. 2012; 32(11): 867–879.
Vijayaraghavan K, Nalini SPK. Biotemplates in the green synthesis of silver nanoparticles. Biotechnol J. 2010; 5: 1098–1110.
Shankar SS, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core–Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Colloid Interface Sci. 2004; 275(2): 496–502.
Sherin L, Sohail A, Amjad US, Mustafa M, Jabeen R, Ul-Hamid A. Facile green synthesis of silver nanoparticles using Terminalia bellerica kernel extract for catalytic reduction of anthropogenic water pollutants. Colloid Interface Sci Commun. 2020; 37: 100276.
Nestor ARV, Mendieta VS, Lopez MAC, Espinosa RMG, Lopez MAC, Alatorre JAA. Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. Mater Lett. 2008; 62: 3103–3105.
Aragão AP, Oliveira TM, Quelemes PV, Perfeito MLG, Araújo MC, Santiago JAS, Cardoso VS, Quaresma P, Leite JRA, Silva DA. Green synthesis of silver nanoparticles using the seaweed Gracilaria Birdiae and their antibacterial activity. Arab J Chem. 2019; 12(8): 4182–4188.
Cvetković DJ, Stanojević LjP, Stanković MZ, Cakić MD, Savić SR, Miljković MD. Antioxidant activity of strawberry (Fragaria × ananassa Duch.) leaves. Sep Sci Technol. 2017; 52: 1039–1051.
Cvetković D, Marković D. UV-effects on antioxidant activity of selected carotenoids in the presence of lecithin estimated by DPPH test. J Serb Chem Soc. 2008; 73(11): 1051–1061.
Stanojević LjP, Zdravković AS, Stanković MZ, Cakić MD, Nikolić VD, Ilić DP. Antioksidativna aktivnost vodeno-etanolnih ekstrakata iz lista koprive (Urtica dioica L.). Savremene tehnologije. 2013; 2(1):51–59. (In Serbian)
Veličković JM, Kostić DA, Stojanović GS, Mitić SS, Mitić MN, Ranđelović SS, Đorđević AS. Phenolic composition, antioxidant and antimicrobial activity of the extracts from Prunus spinosa L. fruit. Hem. ind. 2014; 68(3): 297–303. (In Serbian)
Bednar N. Synthesis of metallic nanoparticles in electrolyte-plasma interface [dissertation]. Novi Sad: University of Novi Sad, Serbia; 2014.
Sabri MA, Umer A, Awan GH, Hassan MF, Hasnain A. Selection of suistable biological method for the synthesis of silver nanoparticles. Nanomater nanotechnol. 2016; 6(29): 1–20.
Ebrahiminezhad A, Barzegar Y, Ghasemi Y, Berenjian A. Green synthesis and characterization of silver nanoparticles using Alcearosea flower extract as a new generation of antimicrobials. Chem Ind Chem Eng Q. 2017; 23(1): 31–37.
Ivanov IG, Vracheva VZ, Marchev AS, Petkova NT, Aneva IY, Danev PP, Georgiev VG. Antioxidant activites and phenolic compounds in Bulgarien Fumaria species. Int J Curr Microbiol App Sci. 2014; 3(2): 296–306.
Kumar V, Yadov SK. Plant‐mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol. 2009; 84: 151–157.
Iacopini P, Baldi M, Storchi P, Sebastiani L. Catechin, epicatechin, quercetin, rutin and resveratrol in red grape: Content, in vitro antioxidant activity and interactions. J Food Compos Anal. 2008; 21(8): 589–598.
Ndhlala AR, Moyo M, Staden JV. Natural antioxidants: Fascinating or mythical biomolecules. Molecules. 2010; 15(10): 6905–6930.
Pouillot A, Polla LL, Tacchini P, Neequaye A, Polla A, Polla B. Natural antioxidants and their effects on the skin, John Wiley Sons Inc. 2011; 239–257.
Orak HH, Yagar H, Isbilir SS, Demirci AS, Gümüş T, Ekinci N. Evaluation of antioxidant and antimicrobial potential of strawberry tree (Arbutus Unedo L.) leaf. Food Sci Biotechnol. 2011; 20(5): 1249–1256.
Seleshe S, Lee JS, Lee S, Lee HJ, Kim GR, Yeo J, Kim JY, Kang SN. Evaluation of antioxidant and antimicrobial activities of ethanol extracts of three kinds of strawberries. Prev Nutr Food Sci. 2017; 22(3): 203–210.
Ravichandran V, Vasanthi S, Shalini S, Shah SAA, Harish R. Green synthesis of silver nanoparticles using Atrocarpus altilis leaf extract and the study of their antimicrobial and antioxidant activity. Mater Lett. 2016; 180: 264–267.
Wang L, Wu Y, Xie J, Wu S, Wu Z. Characterization, antioxidant and antimicrobial activities of green synthesized silver nanoparticles from Psidium guajava L. leaf aqueous extracts. Mater Sci Eng C. 2018; 86: 1–8.
Rasheed T, Bilal M, Iqbal HMN, Li C. Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential biomedical applications. Colloids Surf B. 2017; 158: 408–415.
Kumar DA, Palanichamy V, Roopan SM. Green synthesis of silver nanoparticles using Alternanthera dentata leaf extract at room temperature and their antimicrobial activity. Spectrochim Acta, Part A. 2014; 127: 168–171.
Nabikhan A, Kandasamy K, Raj A, Alikunhi NM. Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L. Colloids Surf B. 2010; 79(2): 488–493.
Zhang Y, Yang D, Kong Y, Wang X, Pandoli O, Gao G. Synergetic antibacterial effects of silver nanoparticles Aloe Vera prepared via a green method. Nano Biomed Eng. 2010; 2(4): 252–257.
Stanojević Lj, Cvetković D, Danilović B, Cakić M, Stanojević D. Antioxidant and antimicrobial activity of aqueous extracts from cultivated strawberry (Fragaria x ananassa Duch.) leaves from Serbia, 24th Congress of Chemists and Technologists of Macedonia, Ohrid, Republic of Macedonia; 2016, p. 212.
Glišić S, Cakić M, Nikolić G, Danilović B. Synthesis, characterization and antimicrobial activity of carboxymethyl dextrane stabilized silver nanoparticles. J Mol Struct. 2015; 1084: 345–351.
Nagajyothi PC, Lee KD. Synthesis of plant-mediated silver nanoparticles using Dioscorea batatas rhizome extract and evaluation of their antimicrobial activities. J Nanomaterials. 2011; 1–7.
Xiu ZM, Ma J, Alvarez PJJ. Differential Effect of Common Ligands and Molecular Oxygen on Antimicrobial Activity of Silver Nanoparticles versus Silver Ions. Environ Sci Technol. 2011; 45(20): 9003–9008.
Li WR, Xie XB, Shi QS, Duan SS, Ouyang YS, Chen YB. Antibacterial effect of silver nanoparticles on Staphylococcus aureus. BioMetals. 2011; 24(1): 135–141.
Barani H, Montazer M, Samadi N, Toliyat T. In situ synthesis of nano silver/lecithin on wool: Enhancing nanoparticles diffusion. Colloids Surf B. 2012; 92: 9–15.
Gutierrez FM, Thi EP, Silverman JM, Oliveira D, Camargo C, Svensson SL, Hoek AV, Sánchez EM, Reiner NE, Gaynor EC, Pryzdial EL, Conway EM, Orrantia E, Ruiz F, Av-Gay Y, Bach H. Antibacterial activity, inflammatory response, coagulation and cytotoxicity effects of silver nanoparticles. Nanomedicine. 2012; 8: 328–336.
Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology. 2007; 18: 225103–225112.
Tamboli DP, Lee DS. Mechanistic antimicrobial approach of extracellularly-synthesized silver nanoparticles against Gram positive and Gram negative bacteria. J Hazard Mater. 2013; 260: 878–884.
Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res. 2000; 52(4): 662–668.
Klaine SJ, Alvarez PJJ, Batley GE, Fernandes TF, Handy RD, Lyon DY, Mahendra SY, Mclaughlin MJ, Lead JR. Nanomaterials in the environment: behavior, fate, bioavailability and effects. Environ Toxicol Chem. 2008; 27(9): 1825–1851.
Hepokur C, Kariper İA, Mısır S, Ay E, Tunoğlu S, Ersez MS, Zeybek U, Kuruca SE, Yaylım İ. Silver nanoparticle/capecitabine for breast cancer cell treatment, Toxicol In Vitro. 2019; 61: 104600.
Parveena A, Kulkarni N, Yalagatti M, Abbaraju V, Deshpande R. In vivo efficacy of biocompatible silver nanoparticles cream for empirical wound healing. J Tissue Viability. 2018; 27: 257–261.
Prociak JP, Grabowska A, Chwastowski J, Majka TM, Banach M. Safety of the application of nanosilver and nanogold in topical cosmetic preparations. Colloids Surf B. 2019; 183: 110416.