Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology Original scientific paper
Article Sidebar
Main Article Content
Abstract
Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391±0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.
Article Details
Issue
Section
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors grant to the Publisher the following rights to the manuscript, including any supplemental material, and any parts, extracts or elements thereof:
- the right to reproduce and distribute the Manuscript in printed form, including print-on-demand;
- the right to produce prepublications, reprints, and special editions of the Manuscript;
- the right to translate the Manuscript into other languages;
- the right to reproduce the Manuscript using photomechanical or similar means including, but not limited to photocopy, and the right to distribute these reproductions;
- the right to reproduce and distribute the Manuscript electronically or optically on any and all data carriers or storage media – especially in machine readable/digitalized form on data carriers such as hard drive, CD-Rom, DVD, Blu-ray Disc (BD), Mini-Disk, data tape – and the right to reproduce and distribute the Article via these data carriers;
- the right to store the Manuscript in databases, including online databases, and the right of transmission of the Manuscript in all technical systems and modes;
- the right to make the Manuscript available to the public or to closed user groups on individual demand, for use on monitors or other readers (including e-books), and in printable form for the user, either via the internet, other online services, or via internal or external networks.
How to Cite
References
M. Dols, M. Remaud-Simeon, R. M. Willemot, M. Vignon, and P. Monsan, "Characterization of the different dextransucrase activities excreted in glucose, fructose, or sucrose medium by Leuconostoc mesenteroides NRRL B- 1299," Appl. Environ. Microbiol., vol. 64, no. 4, pp. 1298–1302, 1998.
F. Paul, E. Oriol, D. Auriol, and P. Monsan, "Acceptor reaction of a highly purified dextransucrase with maltose and oligosaccharides. Application to the synthesis of controlled-molecular-weight dextrans," Carbohydr. Res., vol. 149, no. 2, pp. 433–441, 1986.
A. Majumder, R. K. Purama, and A. Goyal, "An overview of purification methods of glycoside hydrolase family 70 dextransucrase," Indian Journal of Microbiology, vol. 47, no. 3. pp. 197–206, 2007.
M. Lopretti and L. Martinez, "Influence of nitrogen/carbon ratio and complementary sugars on dextransucrase production by Leuconostoc mesenteroides NRRL B512(f).," Process Biochem., vol. 34, no. 9, pp. 879–884, 2002.
H. Neubauer, A. Bauché, and B. Mollet, "Molecular characterization and expression analysis of the dextransucrase DsrD of Leuconostoc mesenteroides Lcc4 in homologous and heterologous Lactococcus lactis cultures," Microbiology, vol. 149, no. 4. pp. 973–982, 2003.
E. C. Gil, A. I. Colarte, A. El Ghzaoui, D. Durand, J. L. Delarbre, and B. Bataille, "A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets," Eur. J. Pharm. Biopharm., vol. 68, no. 2, pp. 319–329, 2008.
S. Patel, A. Majumder, and A. Goyal, "Potentials of Exopolysaccharides from Lactic Acid Bacteria," Indian J. Microbiol., vol. 52, no. 1, pp. 3–12, 2012.
"GLOBAL DEXTRAN MARKET 2019 BY MANUFACTURERS, REGIONS, TYPE AND APPLICATION, FORECAST TO 2024." [Online]. Available: https://www.360researchreports.com/global-dextran-market-13851154. [Accessed: 28-Jan-2021].
J. H. D. Queiroz and F. Maugerio Filho, "Optimization of the production of dextransucrase from Leuconostoc mesenteroides using molasses as carbon source," Rev. Microbiol., vol. 20, no. 2, pp. 150–156, 1989.
M. Santos, A. Rodrigues, and J. A. Teixeira, "Production of dextran and fructose from carob pod extract and cheese whey by Leuconostoc mesenteroides NRRL B512(f)," Biochem. Eng. J., 2005.
C. M. A. Chagas, T. L. Honorato, G. A. S. Pinto, G. A. Maia, and S. Rodrigues, "Dextransucrase production using cashew apple juice as substrate: Effect of phosphate and yeast extract addition," Bioprocess Biosyst. Eng., vol. 30, no. 3, pp. 207–215, 2007.
A. C. Wilkie, K. J. Riedesel, and J. M. Owens, "Stillage characterization and anaerobic treatment of ethanol stillage from conventional and cellulosic feedstocks," Biomass and Bioenergy, vol. 19, no. 2. pp. 63–102, 2000.
J. S. Kim et al., "Development of clean technology in alcohol fermentation industry," J. Clean. Prod., vol. 5, no. 4, pp. 263–267, 1997.
A. Djukić-Vuković, L. Mojović, S. Nikolić, J. Pejin, S. Kocić-Tanackov, and K. Mihajlovski, "Distillery stillage as a new substrate for lactic acid production in batch and fed-batch fermentation," in Chemical Engineering Transactions, 2013, vol. 34, pp. 97–102.
L. R. Rodrigues, J. A. Teixeira, and R. Oliveira, "Low-cost fermentative medium for biosurfactant production by probiotic bacteria," Biochem. Eng. J., vol. 32, no. 3, pp. 135–142, 2006.
P. Nigam and M. Vogel, "Bioconversion of sugar industry by-products-molasses and sugar beet pulp for single cell protein production by yeasts," Biomass and Bioenergy, vol. 1, no. 6, pp. 339–345, 1991.
J. I. Harland, C. K. Jones, and C. Hufford, "Co-products," in Sugar Beet, A. Draycott, Ed. Blackwell Publishing Ltd., 2006, pp. 451–455.
S. Kalogiannis, G. Iakovidou, M. Liakopoulou-Kyriakides, D. A. Kyriakidis, and G. N. Skaracis, "Optimization of xanthan gum production by Xanthomonas campestris grown in molasses," Process Biochem., vol. 39, no. 2, pp. 249–256, 2003.
L. Mojović et al., "The potential for sustainable bioethanol production in Serbia: available biomass and new production approaches," in Materials and processes for energy: communicating current research and technological developments, 2013, pp. 380–392.
K. R. Mihajlovski, N. R. Radovanović, D. N. Veljović, S. S. Šiler-Marinković, and S. I. Dimitrijević-Branković, "Improved β-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1," Ind. Crops Prod., vol. 80, pp. 115–122, Feb. 2016.
M. Dols, M. Remaud-Simeon, and P. F. Monsan, "Dextransucrase production by Leuconostoc mesenteroides NRRL B-1299. Comparison with L. mesenteroides NRRL B-512F," Enzyme Microb. Technol., vol. 20, no. 7, pp. 523–530, 1997.
A. Goyal and S. Katiyar, "Effect of certain nutrients on the production of dextransucrase from Leuconostoc mesenteroides NRRL B-512F," J. Basic Microbiol., vol. 37, no. 3, pp. 197–204, 1997.
P. Bellengier, J. Richard, and C. Foucaud, "Nutritional requirements of Leuconostoc mesenteroides subsp. mesenteroides and subsp. dextranicum for growth in milk," J. Dairy Res., vol. 64, no. 1, pp. 95–103, 1997.
J. L. Uma Maheswar Rao and T. Satyanarayana, "Improving production of hyperthermostable and high maltose-forming α-amylase by an extreme thermophile Geobacillus thermoleovorans using response surface methodology and its applications," Bioresour. Technol., vol. 98, no. 2, pp. 345–352, 2007.
A. Majumder, S. Bhandari, R. K. Purama, S. Patel, and A. Goyal, "Enhanced production of a novel dextran from Leuconostoc mesenteroides NRRL B-640 by response surface methodology," Ann. Microbiol., vol. 59, no. 2, pp. 309–315, 2009.
A. Djukić-Vuković, D. Mladenović, M. Radosavljević, S. Kocić-Tanackov, J. Pejin, and L. Mojović, "Wastes from bioethanol and beer productions as substrates for l(+) lactic acid production - A comparative study," Waste Manag., vol. 48, 2016.
S. Davidović, M. G. Miljković, D. G. Antonović, M. D. Rajilić-Stojanović, and S. I. Dimitrijević-Branković, "Water kefir grain as a source of potent dextran producing lactic acid bacteria," Hem. Ind., vol. 69, no. 6, 2015.
H. M. Tsuchiya et al., "The effect of certain cultural factors on production of dextransucrase by Leuconostoc mesenteroides.," J. Bacteriol., 1952.
M. G. Miljković et al., "Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses," Appl. Biochem. Biotechnol., vol. 180, no. 5, pp. 1016–1027, 2016.
"Association of Official Analytical Chemists (AOAC)," Off. methods Anal. (17th ed.). Gaithersbg. AOAC Int. (Methods 923.03, 925.09, 930.15, 955.04, 960.39)., 2000.
G. L. Miller, "Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar," Anal. Chem., 1959.
P. Boyaval, P. B. Lactic, and L. Lait, "Lactic acid bacteria and metal ions," Lait, vol. 69, no. 2, pp. 87–113, 1989.
S. Šušić et al., Osnovi tehnologije šećera, Drugo izda. Beograd: Jugošećer, 1995.
M. G. Miljković, S. Z. Davidović, S. Kralj, S. S. Šiler-Marinković, M. D. Rajilić-Stojanović, and S. I. Dimitrijević-Branković, "Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate," Hem. Ind., vol. 71, no. 4, 2017.
K. Adinarayana and P. Ellaiah, "Response surface optimization of the critical medium components for the production of alkaline protease by a newly isolated Bacillus sp," J. Pharm. Pharm. Sci., vol. 5, no. 3, pp. 272–278, 2002.
R. K. Purama and A. Goyal, "Effect of Nutrients by One Variable At A Time (OVAT) Approach on the Dextransucrase Production from Leuconostoc mesenteroides NRRL B-640," Internet J. Microbiol., vol. 5, no. 1, 2012.
A. P. Djukić-Vuković, L. V. Mojović, M. S. Vukašinović-Sekulić, S. B. Nikolić, and J. D. Pejin, "Integrated production of lactic acid and biomass on distillery stillage," Bioprocess Biosyst. Eng., vol. 36, no. 9, pp. 1157–1164, 2013.
T. A. Vedyashkina, V. V. Revin, and I. N. Gogotov, "Optimizing the conditions of dextran synthesis by the bacterium Leuconostoc mesenteroides grown in a molasses-containing medium," Appl. Biochem. Microbiol., 2005.
J. Behravan, B. S. Fazly Bazzaz, and Z. Salimi, "Optimization of dextran production by Leuconostoc mesenteroides NRRL B-512 using cheap and local sources of carbohydrate and nitrogen," Biotechnol. Appl. Biochem., vol. 38, no. 3, p. 267, 2003.
M. Helen et al., "Performance of response surface model for increase of dextransucrase production by Leuconostoc mesenteroides FT 054B under different experimental condition," Asian J. Biolological Life Sci., pp. 29–35, 2012.
R. M. A. Abedin, A. M. El-Borai, M. Abo Shall, and S. A. El-Assar, "Optimization and statistical evaluation of medium components affecting dextran and dextransucrase production by Lactobacillus acidophilus ST76480.01," Life Sci. J., vol. 10, no. 1, pp. 1746–1753, 2013.
"No Title." [Online]. Available: https://sdgs.un.org/goals. [Accessed: 28-Jan-2021].