Ultrafiltration as a simple purification method of a water extract of common bean seed as a natural coagulant

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V 72, No 3
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Published: Jul 1, 2020
DOI: https://doi.org/10.2298/HEMIND200304018P
Keywords:
water clarification, coagulation activity, proteins, organic load

Main Article Content

Jelena M. Prodanović
University of Novi Sad, Faculty of Technology Novi Sad, Blvd. cara Lazara 1, 21 000 Novi Sad
Bojana M. Šarić
University of Novi Sad, Institute of Food Technology in Novi Sad, Blvd. Cara Lazara 1, 21000 Novi Sad, Republic of Serbia
Marina B. Šćiban
University of Novi Sad, Faculty of Technology Novi Sad, Blvd. cara Lazara 1, 21 000 Novi Sad
Dragana V. Kukić
University of Novi Sad, Faculty of Technology Novi Sad, Blvd. cara Lazara 1, 21 000 Novi Sad
Vesna M. Vasić
University of Novi Sad, Faculty of Technology Novi Sad, Blvd. cara Lazara 1, 21 000 Novi Sad
Sanja J. Popović
University of Novi Sad, Institute of Food Technology in Novi Sad, Blvd. Cara Lazara 1, 21000 Novi Sad, Republic of Serbia
Mirjana G. Antov
University of Novi Sad, Faculty of Technology Novi Sad, Blvd. cara Lazara 1, 21 000 Novi Sad

Abstract

Natural coagulants from a crude water extract of common bean seed showed very good efficiency of turbidity removal from water of ~89 % under optimal coagulation conditions, which were determined using response surface methodology (RSM). However, they also increased the content of organic matter in treated model water by ~66 %, which is the main drawback of usage of natural coagulants, in general. Thus, ultrafiltration was applied for processing of the crude water extract in order to separate biomolecules, which exhibit the coagulation activity. Four fractions obtained by ultrafiltration were applied in coagulation tests under the same conditions as the crude extract, and the 4th fraction (molecules with molecular weights >30 kDa) with the predominant content of proteins with molecular weights 50 – 60 kDa, achieved almost as high efficiency of turbidity removal (75 %) as the crude extract. At the same time, the content of organic matter in treated water increased just for 16 % in comparison to the blank (model water processed in the same way but without coagulant). After optimization of process parameters by RSM for usage of the 4th fraction, the coagulation activity increased further to 80 %.

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How to Cite
Prodanović, J. M., Šarić, B. M., Šćiban, M. B., Kukić, D. V., Vasić, V. M., Popović, S. J., & Antov, M. G. (2020). Ultrafiltration as a simple purification method of a water extract of common bean seed as a natural coagulant. HEMIJSKA INDUSTRIJA (Chemical Industry), 74(3), 211–220. https://doi.org/10.2298/HEMIND200304018P
Issue
Vol. 74 No. 3 (2020)
Section
Environmental Engineering - Waste Water Treatment
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