Ultrafiltration as a simple purification method of a water extract of common bean seed as a natural coagulant

Jelena M. Prodanović, Bojana M. Šarić, Marina B. Šćiban, Dragana V. Kukić, Vesna M. Vasić, Sanja J. Popović, Mirjana G. Antov


Natural coagulants from a crude water extract of common bean seed showed very good efficiency of turbidity removal from water of ~89 % under optimal coagulation conditions, which were determined using response surface methodology (RSM). However, they also increased the content of organic matter in treated model water by ~66 %, which is the main drawback of usage of natural coagulants, in general. Thus, ultrafiltration was applied for processing of the crude water extract in order to separate biomolecules, which exhibit the coagulation activity. Four fractions obtained by ultrafiltration were applied in coagulation tests under the same conditions as the crude extract, and the 4th fraction (molecules with molecular weights >30 kDa) with the predominant content of proteins with molecular weights 50 – 60 kDa, achieved almost as high efficiency of turbidity removal (75 %) as the crude extract. At the same time, the content of organic matter in treated water increased just for 16 % in comparison to the blank (model water processed in the same way but without coagulant). After optimization of process parameters by RSM for usage of the 4th fraction, the coagulation activity increased further to 80 %.


water clarification; coagulation activity; proteins; organic load


Becaria A, Lahiri DK, Bondy SC, Chen D-M, Hamadeh A, Li H, Taylor R, Campbell A. Aluminium and copper in drinking water enhance inflammatory or oxidative events specifically in the brain. J Neuroimmunol. 2006; 176: 16-23.

Walton JR, Wang M-X. APP expression, distribution and accumulation are altered by aluminium in a rodent model for Alzheimer’s disease. J Inorg Biochem. 2009; 103: 1548-1554.

Bondy SC. The neurotoxicity of environmental aluminium is still an issue. Neurotoxicology. 2010; 31: 575-581.

Chico Galdo V, Massart C, Jin L, Vanvooren V, Caillet-Fauquet P, Andry G, Lothaire P, Dequanter D, Friedman M, Van Sande J. Acrylamide, an in vivo thyroid carcinogenic agent, induces DNA damage in rat thyroid cell lines and primary cultures. Mol Cell Endocrinol. 2006; 257-258: 6-14.

Ndabigengesere A, Narasiah KS. Quality of water treated by coagulation using Moringa oleifera seeds. Water Res. 1998; 32: 781-791.

Okuda T, Baes AU, Nishijima W, Okada M. Isolation and characterization of coagulant extracted from Moringa oleifera seed by salt solution. Water Res. 2001; 35: 405-410.

Beltrán-Heredia J, Sánchez-Martín J, Delgado-Regalado A, Jurado-Bustos C. Removal of Alizarin Violet 3R (anthraquinonic dye) from aqueous solutions by natural coagulants. J Hazard Mater. 2009; 170: 43-50.

Beltrán-Heredia J, Sánchez-Martín J. Removal of sodium lauryl sulphate by coagulation/flocculation with Moringa oleifera seed extract. J Hazard Mater. 2009; 164: 713-719.

Ghebremichael K, Abaliwano J, Amy G. Combined natural organic and synthetic inorganic coagulants for surface water treatment. Journal of Water Supply: Research and Technology – AQUA. 2009; 58: 267-276.

Šćiban M, Klašnja M, Stojimirović J. Investigation of coagulation activity of natural coagulants from seeds of different Leguminose species. Acta Period Technol. 2005; 36: 81-87.

Šćiban MB, Vasić MA, Prodanović JM, Antov MG, Klašnja MT. The investigation of coagulation activity of natural coagulants extracted from different strains of common bean. Acta Period Technol. 2010; 41:141-147.

Antov MG, Šćiban MB, Petrović NJ. Proteins from common bean (Phaseolus vulgaris) seed as a natural coagulant for potential application in water turbidity removal. Bioresour Technol. 2010; 101: 2167-2172.

Prodanović JM, Šćiban MB, Antov MG, Dodić JM. Comparing the use of common bean extracted natural coagulants with centrifugation in the treatment of distillery wastewaters. Rom. Biotechnol. Lett. 2011; 16: 6638-6647.

Vasić VM, Prodanović JM, Kukić DV, Šćiban MB, Antov MG, Ivetić DŽ. Application of membrane and natural coagulants for stillage purification. Desalin Water Treat. 2013; 51: 437-441.

Muyibi SA, Noor MJMM, Tan KL, Lam HL. Effects of oil extraction from Moringa oleifera seeds on coagulation of turbid water. Int J Environ Stud. 2002; 59: 243-254.

Ghebremichael KA, Gunaratna KR, Dalhammar G. Single-step ion exchange purification of the coagulant protein from Moringa oleifera seed. Appl Microbiol Biotechnol. 2006; 70: 526-532.

Antov MG, Šćiban MB, Prodanović JM. Evaluation of the efficiency of natural coagulant obtained by ultrafiltration of common bean seed extract in water turbidity removal. Ecol Eng. 2012; 49: 48-52.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227: 680-685.

Nkurunziza T, Nduwayezu JB, Banadda EN, Nhapi I. The effect of turbidity levels and Moringa oleifera concentration on the effectiveness of coagulation in water treatment. Water Sci Technol. 2009; 59: 1551-1558.

Pritchard M, Mkandawire T, Edmondson A, O’Neill JG, Kululanga G. Potential of using plant extracts for purification of shallow well water in Malawi. Phys Chem Earth. 2009; 34: 799-805.

Pritchard M, Craven T, Mkandawire T, Edmondson AS, O’Neill JG. A study of the parameters affecting the effectiveness of Moringa oleifera in drinking water purification. Phys Chem Earth. 2010; 35: 791-797.

Feliks R, Škunca-Milovanović S. Voda za piće. Standardne metode za ispitivanje higijenske ispravnosti. Beograd, Savezni zavod za zdravstvenu zaštitu & NIP Privredni pregled; 1990: 134-136. (in Serbian)

Pritchard M, Craven T, Mkandawire T, Edmondson AS, O’Neill JG. A comparison between Moringa oleifera and chemical coagulants in the purification of drinking water – An alternative sustainable solution for developing countries. Phys Chem Earth. 2010; 35: 798-805.

Ghebremichael KA, Gunaratna KR, Henriksson H, Brumer H, Dalhammar G. A simple purification and activity assay of the coagulant protein from Moringa oleifera seed. Water Res. 2005; 39: 2338-2344.

Montoya CA, Lallès J-P, Beebe S, Leterme P. Phaseolin diversity as a possible strategy to improve the nutritional value of common beans (Phaseolus vulgaris). Food Res Int. 2010; 43: 443-449.

Montoya CA, Leterme P, Victoria NF, Toro O, Souffrant WB, Beebe S, Lallès J-P. The susceptibility of phaseolin to in vitro proteolysis is highly variable across Phaseolus vulgaris bean varieties. J Agric Food Chem. 2008; 56: 2183-2191.

DOI: https://doi.org/10.2298/HEMIND200304018P

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