Stabilizacija magnetnih čestica polianilinom i imobilizacija alfa-amilaze
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Abstract
U ovom radu sintetisane su magnetne čestice metodom koprecipitacije, a zatim je izvršena polimerizacija anilina na njihovoj površini. Dobijene čestice upotrebljene su kao nosač za imobilizaciju α-amilaze iz Bacillus licheniformis. FTIR spektroskopskom analizom pokazane supromene spektara magnetnih česticausled stvaranja polianilinske prevlake i imobilizacije α-amilaze na magnetnim česticama sa i bez polimernog sloja. Kod magnetnih čestica je prisutna bimodalna, a kod magnetnih čestica sa polianilinskom prevlakom trimodalna raspodela veličine čestica. Merenjem zeta potencijala magnetnih čestica sa i bez polianilina utvrđeno je da dolazi do stabilizacije čestica nakon stvaranja polianilinske prevlake. Oblaganje magnetnih čestica polianilinom doprinelo je i poboljšanju kinetičkih svojstava imobilisanog enzima, jer jeMihaelisova konstantahidrolize skroba, Km imobilisane α-amilaze na magnetnim česticama 1,91 g L-1 i imobilisane α-amilaze na magnetnim česticama presvučenim polianilinom 1,48 g L-1. Adsorpcija enzima na magnetnim česticama presvučenim polianilinomdoprinela je pH i temperaturnoj stabilizaciji α-amilaze. U protočnom reaktoru sa pakovanim slojem sa povratnim tokom u prvom ciklusu već nakon 20 min hidrolizovalo je 98,8% skroba, dok je tokom petog ciklusa bio potreban približno pet puta duži vremenski period da hidrolizuje 93,5% skroba. U kontinualnim uslovima rada reaktora stepen hidrolize skroba se nije menjao značajnije u toku 4 h i iznosio je 88,8±1,6%, a vreme polu-života biokatalizatora bilo je 6,2 h.
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