Antibiotic production by Streptomyces hygroscopicus CH-7 in medium containing Schiff base complexes

Slavica B Ilić, Sandra S Konstantinović, Gordana Dj Gojgić-Cvijović, Vlada B Veljković

Abstract


Influence of a modified media on Hexaene H-85 and Azalomycine B production by Streptomyces hygroscopicus CH-7 was investigated. The amino acid L-tryptophan, as a nitrogen source, was replaced with isatin-3-thiosemicarbazone and its complexes with some divalent metal ions. Isatin-3-thiosemicarbazone was synthesized in crude glycerol obtained as a byproduct in biodiesel production from sunflower oil. The complexes were charac­terized by elemental microanalysis and magnetic susceptibility, as well as, by Atomic absorption(AA), Fourier-transform infrared (FTIR) and Ultraviolet–visible (UV/VIS) spectro­scopic methods. The spectral studies indicated an octahedral geometry for the Mn(II), Fe(II) and Ni(II) complexes and a tetrahedral one for the Zn(II) complex. Comparing to the basal medium, isatin-3-thiosemicarbazone (ITC) and its metal complexes in the concentration of 0.5 g dm-3 showed better results in the antibiotics production. Use of medium supple­mented with the Fe(II) complex resulted in the maximum Hexaene H-85 and Azalomycine B concentrations of 306 µg cm-3 and 127 µg cm-3,respectively. Addition of ITC and its complexes changed the morphology of S. hygroscopicus CH-7 from filaments to pellets as a dominant shape in media resulting in higher antibiotic production.

Keywords


antibiotic production; isatin-3-thiosemicarbazone; metal complexes morphology; Streptomyces hygroscopicus

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References


Berdy J. Bioactive Microbial Metabolites. J Antibiot. 2005; 58: 1-26.

Watve MG, Tickoo R, Jog MM, Bhole BD. How many antibiotics are produced by the genus Streptomyces?.Arch Microbiol. 2001; 176: 386-390.

Ilić SB, The effect of composition and rheology of nutrition medium on kinetic of antibiotic production by bacteria Streptomyces hygroscopicus CH-7, Ph.D. Thesis, Faculty of Technology, University of Nis, Leskovac, 2010.

Haferburg G, Kothe E. Microbes and metals: interactions in the environment. J Basic Microbiol. 2007; 47: 453-467.

Schrader KK, Blevins WT. Effects of carbon source, phosphours concentration, and several micronutrients on biomass and geosmin production by Streptomyces halstedii. J Ind Microb Biotech. 2001; 26: 241-247.

Thanh HL, Nga TV, Thuy TH, Tam BKN, Van HTD. Effects of some microelements on antifungal activity and biomass of the Actinomyces producing Validamycin-A. J Viet Env. 2018; 9:128-131.

Bundale S, Begde D, Nashikkar N, Kadam T, Upadhyay A. Optimization of Culture Conditions for Production of Bioactive Metabolites by Streptomyces spp. Isolated from Soil. Adv Microb. 2015; 5: 441-451.

Ripa FA, Nikkon F, Zaman S, Khondkar P. Optimal Conditions for Antimicrobial Metabolites Production from a New Streptomyces sp. RUPA-08PR Isolated from Bangladeshi Soil. Mycobiology. 2009; 37: 211-214.

Hassan MA, El-Naggar MY, Said WY. Physiological factors affecting the production of an antimicrobial substance by Streptomyces violatus in batch cultures. Egypt J Biology. 2001; 3: 1-10.

Majzlik P, Strasky A, Adam V, Nemec M, Trnkova L, ZehnalekHubalek J, Provaznik I, KizekR.Influence of zinc (II) and copper (II) ions on Streptomyces bacteria revealed by electrochemistry. Int J ElectrochemSci. 2011; 6: 2171-2191.

Cai P, Kong F, Fink P, Ruppen ME, Williamson RT, Keiko T. Polyene antibiotics from Streptomyces mediocidicus. J Nat Prod. 2007; 70:215–219.

Karadzic I, Gojgic-Cvijovic G, VučetićJ.Hexaene H-85, A hexaene H-85 macrolide complex. J Antibiot. 1991; 12: 1452-1453.

Kaiser H, Keller-Schierlein W. Metabolites of microorganisms. Part 202. Structure elucidation of elaiophylin:spectroscopic studies and degradation. HelvChim Acta. 1981; 64:407-424.

Banga J, Praveen V, Singh V, Tripathi CKM, Bihari V. Studies on medium optimization for the production of antifungal and antibacterial antibiotics from a bioactive soil actinomycete. Med Chem Res. 2008; 17: 425-436.

Mahesh M, Meenakshi N. Effect of carbon and nitrogen source for the production of tetracycline analysis by using HPLC. Int J Adv Biotechnol Res. 2013; 4: 218–223.

Ćirić JT, Konstantinović SS, Ilić SB, Gojgić-Cvijović G, Savić DS, Veljković VB. The impact of isatin derivatives on antibiotic production by Streptomyces hygroscopicus CH-7. Hem Ind. 2016; 70: 123-128.

Ilić SB,KonstantinovićSS,Gojgić-Cvijović G, Savić DS, Veljković VB. The influence of Schiff base inclusion complexes with β-cyclodextrine on antibiotic production by Streptomyces hygroscopicus CH-7. Hem Ind. 2015; 69:9-15.

Konstantinović SS, Zlatković MZ, ĆirićJT,Ilić SB, Gojgić-Cvijović G, Veljković VB. The use salicylaldehyde derivatives as a nitrogen sources for antibiotic production by Streptomyces hygroscopicus CH-7. Hem Ind. 2017; 71:487-494.

Isahak WN, Ismail M, Yarmo YA, JahimJM, SalimonJ.Purification of Crude Glycerol from Transesterification RBD Palm over Homogeneous and Heterogeneus Catalysts for the Biolubricant Preparation. J App Sci. 2010; 10: 2590-2595.

Konstantinovic SS, Radovanovic BC, Krkljes A. Thermal behaviour of Co(II), Ni(II), Cu(II), Zn(II), Hg(II) and Pd(II) complexes with isatin-β-thiosemicarbazone. J Therm Anal Calorim. 2007; 90: 525–531.

Vucetic J, Karadzic I, Gojgic-Cvijovic G, Radovanovic E. Improving hexaene H-85 production by Streptomyces hygroscopicus. J Serb Chem Soc. 1994; 59: 973-980.

Ilic SB, Konstantinovic SS, Savic DS, Veljkovic VB, Gojgic-Cvijovic G. The impact of Schiff bases on antibiotic production by Streptomyces hygroscopicus. Med Chem Res. 2010; 19: 690-697.

Ilic SB, Konstantinovic SS, Gojgic-Cvijovic G, SavicDS,Veljkovic VB. The impact of glycerol and some carbohydrates on antibiotic production by Streptomyces hygroscopicus CH-7. Med Chem Res. 2013; 22: 934-937.

Arai M. Azalomycins B and F. Two new antibiotics. J Antibio Ser A. 1960; 13: 51-56.

Temperini MLA, dos Santos MR, Paoli Monteiro VR.Spectroscopic study of the isomerization of Z- to E-pyridine-2-formyl thiosemicarbazone. Spectrochim Acta, Part A, 1995; 51: 1517-1524.

Abdulghani AJ, Hussain RK. Synthesis and Characterization of Schiff Base Metal Complexes Derived from Cefotaxime with 1H-indole-2,3-dione (Isatin) and 4-N,N-dimethyl-aminobenzaldehyde. Open J Inor Chem. 2015; 5: 83-101.

Ferrari MB, Pelizzi C, Pelosi G, Rodrigez-Arguelles MC. Preparation, characterization and X-ray structures of 1-methylisatin 3-thiosemicarbazone copper, nickel and cobalt complexes. Polyhedron. 2002; 21: 2593–2599.

Mohanan K, MurakanB.Complexes of Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II) with a bishydrazone. Synth React Inorg Met Org Chem. 2005; 35: 837–844.

Konstantinovic SS, Synthesis, structure and antimicrobial activity of coordination compounds of isatin Schiff base, Ph. D. Thesis, Faculty of Mathematics and Natural Science, University of Nis, October 2007.

Konstantinovic SS, Radovanovic BC, Sovilj SP, Stanojevic SS. Antimicrobial activity of some isatin-3-thiosemicarbazone complexes. J Serb Chem Soc. 2008; 72: 7-13.

Podunavac-Kuzmanović SO, Leovac VM, Perišić-Janjić NU, Rogan J, Balaž G. Complexes of Cobalt(II), Zinc(II) and Copper(II) with Some Newly Synthesized Benzimidazole Derivatives and Their Antibacterial Activity. J Serb Chem Soc. 1999; 64: 381-388.

Hamedi J, Nobary SG. Effect of single additive bivalent metal ions on the growth of Streptomyces clavuligerus and clavulanic acid production. Prog Biolog Sci. 2012; 3: 24-30.

Kishimoto K, Park YS, Okabe M, Akiyama S. Effect of ferrous ion on amino acid metabolism in mildiomycin production by Streptoverticilliumrimofaciens. J Antib. 1997; 50: 206-211.

Mansour FA, El-Shirbiny SA, El-Metwaly NA. Demethyltetracycline biosynthesis by Streptomyces aureofaciens Sub-species viridulans as influenced by medium composition. Egypt J Microbiol. 1996; 31: 221-235.

Gesheva V, Ivanova V, Gesheva R. Effects of nutrients on the production of AK-111-811 macrolide antibiotic by Streptomyces hygroscopicus. Microbiol Res. 2005; 160: 243-248.




DOI: https://doi.org/10.2298/HEMIND181016008I

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