Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate
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Abstract
The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 °C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K2HPO4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 °C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca2+, Mg2+, Fe2+ and Co2+ led to a minor increase of DS activity, while the addition of Mn2+ was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.
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P. Ruas-Madiedo, J. Hugenholtz, P. Zoon, An overview of the functionality of exopolysaccharides produced by lactic acid bacteria, Int. Dairy J. 12 (2002) 163–171.
H. Leemhuis, T. Pijning, J.M. Dobruchowska, S.S. van Leeuwen, S. Kralj, B.W. Dijkstra, L. Dijkhuizen, Glucan-sucrases: Three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications, J. Biotechnol. 163 (2013) 250–272.
A. Majumder, R.K. Purama, A. Goyal, An overview of purification methods of glycoside hydrolase family 70 dextransucrase, Indian J. Microbiol. 47 (2007) 197–206.
A. Jeanes, W.C. Haynes, C.A. Wilham, J.C. Rankin, E.H. Melvin, M.J. Austin, J.E. Cluskey, B.E. Fischer, H.M. Tsuchiya, C.E. Rist, Characterization and classification of dextrans from ninety-six strains of bacteria, J. Am. Chem. Soc. 78 (1954) 2499–2502.
J.F. Robyt, Dextran, in: H.F. Mark (Ed.), Encyclopedia of Polymer Science and Engineering, 2nd ed., John Wiley & Sons, New York, 1986, pp. 752–767.
F. Paul, E. Oriol, D. Auriol, P. Monsan, Acceptor reaction of a highly purified dextransucrase with maltose and oligosaccharides. Application to the synthesis of controlled-molecular-weight dextrans, Carbohydr. Res. 149 (1986) 433–441.
R. Mehvar, Dextrans for targeted and sustained delivery of therapeutic and imaging agents, J. Control Release 69 (2000) 1–25.
M. Naessens, A. Cerdobbel, W. Soetaert, E. Vandamme, Leuconostoc dextransucrase and dextran: production, properties and applications, J. Chem. Technol. Bio-technol. 80 (2005) 845–860.
G. Lacaze, M. Wick, S. Cappelle, Emerging fermentation technologies: Development of novel sourdoughs, Food Microbiol. 24 (2007) 155–160.
R. Sen, Biotechnology in petroleum recovery: The microbial EOR, Prog. Energy Combust. 34 (2008) 714–724.
S.J.F. Vincent, E.J. Faber, J.R. Neeser, F. Stingele, J.P. Kamerling, Structure and properties of the expolysaccharide produced by Streptococcus macedonicus Sc 136, Glycobiology 11 (2001) 131–139.
P.T. Murphy, R.L. Whistler, Dextrans, in: R.L. Whistler, J.N. BeMiller (Eds.), Industrial Gums. Polysaccharides and Their Derivatives, 2nd ed., Academic Press, New York, 1973, pp. 513–542.
R.K. Purama, A. Goyal, Screening and optimization of nutritional factors for higher dextransucrase production by Leuconostoc mesenteroides NRRL B-640 using statistical approach, Bioresource Technol. 99 (2008) 7108–7114.
S. Patel, D. Kothari, A. Goyal, Purification and characterization of an extracellular dextransucrase from Pediococcus pentosaceus isolated from the soil of northeast India, Food Technol. Biotechnol. 49 (2011) 297–303.
E.J. Vandamme, C.E.F.G. Renard, F.R.J. Arnaut, N.M.F. Vekemans, P.P.A. Tossut, Process for obtaining improved structure build-up of baked products, European Patent, EP 0 790 003A1, 1997.
E.J. Vandamme, C.E.F.G. Renard, F.R.J. Arnaut, N.M.F. Vekemans, P.P.A. Tossut, Process for obtaining improved structure build-up of baked products, US Patent 6 399 119, 2002.
S. Davidović, M. Miljković, D. Antonović, M. Rajilić-Stojanović, S. Dimitrijević-Branković, Water kefir grain as a source of potent dextran producing lactic acid bacteria, Hem. Ind. 69 (2015) 595–604.
H.M. Tsuchiya, H.J. Koepsell, J. Corman, M.O. Bryant, V.H. Geger, R.W. Jackson, The effect of certain cultural factors on production of dextransucrase by Leuconostoc mesenteroides, J. Bacteriol. 64 (1952) 521–526.
G.L. Miller, Use of dinitrosalicylic acid reagent for determination of reducing sugars, Anal. Chem. 31 (1959) 426–429.
O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193 (1951) 265–275.
A. Goyal, S.S. Katiyar, Fractionation of Leuconostoc mesenteroides NRRL B-512F dextransucrase by polyethylene glycol: a simple and effective method of purification, J. Microbiol. Methods 20 (1994) 225–231.
U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680–685.
S.M. Holt, H. Al-Sheikh, K.J. Shin, Characterization of dextran producing Leuconostoc strains, Lett. Appl. Microbiol. 32 (2001) 185–189.
R.K. Purama, A. Goyal, Identification, effective purification and functional characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-640, Biores. Technol. 99 (2008) 3635–3642.
A. Goyal, M. Nigan, S.S. Katiyar, Optimal conditions for production of dextransucrase from Leuconostoc mesenteroides NRRL B512 and its properties, J. Basic Microb. 36 (1995) 375–384.
M. Cortezi, R. Monti, J. Contiero , Temperature effect on dextransucrase production by Leuconostoc mesenteroides FT 045 B isolated from Alcohol and Sugar Mill Plant, Afr. J. Biotechnol. 4 (2005) 279–285.
M. Santos, A. Rodrigues, J. Teixeira, Production of dextran and fructose from carob pod extract and cheese whey by Leuconostoc mesenteroides NRRL B512(f), Biochem. Eng. J. 25 (2005) 1–6.
M. Lopretti, E. Martinez, L. Torres, R. Perdomo, M. Santos, A.E. Rodrigues, Influence of nitrogen:carbon ratio and complementary sugars on dextransucrase product-ion by Leuconostoc mesenteroides NRRL B512(f), Process Biochem. 34 (1999) 879–884.
R.K. Purama, A. Goyal, Effect of nutrients by one variable at a time (OVAT) approach on the dextransucrase production from Leuconostoc mesenteroides NRRL B-640, Internet J. Microbiol. 5 (2007).
S. Shukla, A. Goyal, Optimization of fermentation medium for enhanced glucansucrase and glucan production from Weissella confuse, Braz. Arc. Biol. Technol. 54 (2011) 1117–1124.
D. Das, A. Goyal, Isolation, purification and functional characterization of glucansucrase from probiotic Lactobacillus plantarum DM5, Ann. Microbiol. 64 (2014) 1715–1724.
S.A. Ul-Qader, L. Iqbal, H.A. Rizvi, R. Zuberi, Production of dextran from sucrose by a newly isolated strain of Leuconostoc mesenteroides (PCSIR-3) with reference to Leuconostoc mesenteroides NRRL B-512 F, Biotechnol. Appl. Biochem. 34 (2001) 93–97.
A. Goyal, S.S. Katiyar, Effect of certain nutrients on the production of dextransucrase from Leuconostoc mesenteroides NRRL B-512F, J. Basic Microbiol. 37 (1997) 197––204.
Z. Xia, Effect of Tween 80 on the production of curdlan by Alcaligenes faecalis ATCC 31749, Carbohyd. Polym. 98 (2013) 178–180.
R.K. Purama, A. Goyal, Dextransucrase production by Leuconostoc mesenteroides, Indian J. Microbiol. 45 (2005) 89–101.
A. Majumder, A. Mangtani, A. Goyal, Purification, identification and functional characterization of glucansucrase from Leuconostoc dextranicum NRRL B-1146, Curr. Trends Biotechnol. Pharm. 2 (2008) 493–505.
M. Chellapandian, C. Larios, M. Sanchez-Gonzalez, A. Lopez-Munguia, Production and properties of a dextransucrase from Leuconostoc mesenteroides IBT-PQ isolated from "pulque", a traditional Aztec alcoholic beverage, J. Ind. Microbiol. Biot. 21 (1998) 51–56.
J.P. Martinez-Espindola, A. Lopez-Munguia, On the kinetics of dextransucrase and dextran synthesis in batch reactors, Biotechnol. Lett. 7 (1985) 483–486.
R.K. Purama, M. Agrawal, A. Goyal, Stabilization of dextransucrase from Leuconostoc mesenteroides NRRL B-640, Indian J. Microbiol. 50 (2010) S57–S61.
A.W. Miller, J.F. Robyt, Stabilization of dextransucrase from Leuconostoc mesenteroides NRRL B-512 F by non-ionic detergents, poly (ethylene glycol) and high molecular weight dextran, Biochim. Biophys. Acta 785 (1984) 89–96.
M. Kobayashi, K. Matsuda, Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299, J. Biochem. 79 (1976) 1301-1308.
Y. Yalin, L. Jin, W. Jianhua, T. Da, T. Zigang, Expression and characterization of dextransucrase gene dsrX from Leuconostoc mesenteroides in Escherichia coli, J. Biotechnol. 133 (2008) 505–512.
A. Majumder, A. Goyal, Optimization of culture condi-tions of a novel glucan producing glucansucrase from Leuconostoc dextranicum NRRL B-1146, Curr. Trends Biotechnol. Pharm. 2 (2008) 260–268.