β-glucan-enriched fraction from mosaic puffball induces inflammation in an in vitro 3D bovine chondrocytes model Abstract
Main Article Content
Abstract
Fungal β-glucans are well-known for their immunomodulatory activity. They act as pathogen-associated molecular patterns (PAMPs) and can bind to a number of pattern-recognition receptors (PRRs). Activation of PRRs leads to inflammatory response and, although these receptors are primarily found in immune cells, chondrocytes express certain types of PRRs as well (toll-like receptors –TLRs). Although β-glucans are primarily considered immunestimulatory agents, recent research found that they may have beneficial effects in some inflammatory conditions (hence the term “immunomodulators”), in a complex way that is yet to be uncovered. The aim of this study was to investigate if the mushroom β-glucans could induce any changes in metabolic activity and phenotype of bovine chondrocytes, using a 3D cell culture model. For this purpose, glucan-enriched extract of mosaic puffball fruiting bodies, containing up to 70% (1→6)(1→3)β-D-glucan-protein complex was used.
Article Details
Section
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors grant to the Publisher the following rights to the manuscript, including any supplemental material, and any parts, extracts or elements thereof:
- the right to reproduce and distribute the Manuscript in printed form, including print-on-demand;
- the right to produce prepublications, reprints, and special editions of the Manuscript;
- the right to translate the Manuscript into other languages;
- the right to reproduce the Manuscript using photomechanical or similar means including, but not limited to photocopy, and the right to distribute these reproductions;
- the right to reproduce and distribute the Manuscript electronically or optically on any and all data carriers or storage media – especially in machine readable/digitalized form on data carriers such as hard drive, CD-Rom, DVD, Blu-ray Disc (BD), Mini-Disk, data tape – and the right to reproduce and distribute the Article via these data carriers;
- the right to store the Manuscript in databases, including online databases, and the right of transmission of the Manuscript in all technical systems and modes;
- the right to make the Manuscript available to the public or to closed user groups on individual demand, for use on monitors or other readers (including e-books), and in printable form for the user, either via the internet, other online services, or via internal or external networks.
How to Cite
Funding data
-
Horizon 2020
Grant numbers 952033
References
Li, K., Zhu, Y., Zhang, P., Alini, M., Grad, S., & Li, Z. (2023). Anti-inflammatory and pro-anabolic effects of 5-aminosalicylic acid on human inflammatory osteoarthritis models. J Orthop Translat. 2023; 38: 106-116. https://doi.org/10.1016/j.jot.2022.10.003