Whey protein isolate: a versatile dairy-derived hydrogel for bone and vascular tissue engineering and antimicrobial applications Abstract
Article Sidebar
Main Article Content
Abstract
Whey Protein Isolate (WPI) is an inexpensive by-product of the dairy industry, available in large quantities and used as a dietary supplement. WPI is over 97% protein; three-quarters is beta-lactoglobulin (β-LG). WPI in cell culture medium promoted the proliferation and differentiation of bone-forming cells. Solutions of WPI can be heated to form hydrogels, which withstand sterilization by autoclaving; an important practical advantage. Denaturation of β-LG leads to increased hydrophobic interactions and disulphide bond formation and thus crosslinking to form the polymer hydrogel network. We have studied WPI hydrogels as scaffolds for bone-forming cells and carriers of hydrophobic substances.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree to the following terms:Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors grant to the Publisher the following rights to the manuscript, including any supplemental material, and any parts, extracts or elements thereof:
- the right to reproduce and distribute the Manuscript in printed form, including print-on-demand;
- the right to produce prepublications, reprints, and special editions of the Manuscript;
- the right to translate the Manuscript into other languages;
- the right to reproduce the Manuscript using photomechanical or similar means including, but not limited to photocopy, and the right to distribute these reproductions;
- the right to reproduce and distribute the Manuscript electronically or optically on any and all data carriers or storage media – especially in machine readable/digitalized form on data carriers such as hard drive, CD-Rom, DVD, Blu-ray Disc (BD), Mini-Disk, data tape – and the right to reproduce and distribute the Article via these data carriers;
- the right to store the Manuscript in databases, including online databases, and the right of transmission of the Manuscript in all technical systems and modes;
- the right to make the Manuscript available to the public or to closed user groups on individual demand, for use on monitors or other readers (including e-books), and in printable form for the user, either via the internet, other online services, or via internal or external networks.
How to Cite
References
Keppler J. K. et al., Differences in heat stability and ligand binding among β-lactoglobulin genetic variants A, B and C using (1)H NMR and fluorescence quenching Biochim Biophys Acta 2014;1844(6):1083-93
Douglas, T.E.L et a., Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells J Dairy Sci. 2018; 101(1):28-36
Orlien V., Utilizing High Pressure Processing to Induce Structural Changes in Dairy and Meat Products, Reference Module in Food Science 2016, First Edition, 1–9
Norris, K. et al. Marine-inspired enzymatic mineralization of dairy-derived whey protein isolate (WPI) hydrogels for bone tissue regeneration Marine Drugs 2020; 18, 6-18.
Genç, H. et al. Endothelialization of Whey Protein Isolate-Based Scaffolds for Tissue Regeneration, Molecules 2023, 28(20), 7052
Dziadek, M. et al. Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering, Mater Des 2021; 205, 109749
Dziadek, M. et al. Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering, J Biomed Mater Res A 2019; 107, 2479-2491.
Gupta, D. et al. Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration, Mater Des 2020; 188, 108408
Klimek K. et al. Could Curdlan/Whey Protein Isolate/Hydroxyapatite Biomaterials Be Considered as Promising Bone Scaffolds?—Fabrication, Characterization, and Evaluation of Cytocompatibility towards Osteoblast Cells In Vitro, Cells 2022; 11(20), 3251
Platania V. et al. Phloroglucinol-enhanced whey protein isolate hydrogels with antimicrobial activity for tissue engineering, Mater Sci Eng C 2021; 129, 112412
Baines, D., et al. The enrichment of whey protein isolate hydrogels with Poly-γ-glutamic acid promotes the proliferation and osteogenic differentiation of pre-osteoblasts, Gels, accepted 19/12/2023