Whey protein isolate: a versatile dairy-derived hydrogel for bone and vascular tissue engineering and antimicrobial applications Abstract
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Abstract
Whey Protein Isolate (WPI) is an inexpensive by-product of the dairy industry, available in large quantities and used as a dietary supplement. WPI is over 97% protein; three-quarters is beta-lactoglobulin (β-LG). WPI in cell culture medium promoted the proliferation and differentiation of bone-forming cells. Solutions of WPI can be heated to form hydrogels, which withstand sterilization by autoclaving; an important practical advantage. Denaturation of β-LG leads to increased hydrophobic interactions and disulphide bond formation and thus crosslinking to form the polymer hydrogel network. We have studied WPI hydrogels as scaffolds for bone-forming cells and carriers of hydrophobic substances.
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