Direct ultrasound-assisted extraction and characterization of phenolic compounds from fresh houseleek (Sempervivum marmoreum L.) leaves
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The effects of ultrasound power and frequency on the yield of total extractive substances (TES), total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity (AOA) of fresh houseleek leaves extracts obtained by direct ultrasound-assisted extraction (DUAE) were studied. Preliminary extraction of plant material was performed using methanol, acetone and 2-propanol by Soxhlet extraction. It was found that maximum TES yield could be obtained by methanol extraction (2.91±0.02), followed by acetone and 2-propanol with a TES yield of 2.32±0.01 and 2.01±0.03 g per 100 g of fresh plant material, respectively. In the fresh houseleek leaves extracts obtained by DUAE and methanol as the chosen solvent, TPC, TFC and AOA were in the ranges of: 40.5-85.9 mg gallic acid/g dry extract, 12.7-19.3 mg rutin/g dry extract and 24.6-108.2µg/ml, respectively. The results showed that the increase in the ultrasound power and extraction time have positive and significant (p<0.05) effects on the TPC, TFC and AOA, while the increase in the ultrasound frequency leads to a decrease in the TPC, TFC and AOA of the extracts. A chromatographic analysis of crude extract identified the following: Kaempferol 3-O-(6-O-malonylglucoside)-7-O-glucosyde, Kaempferol 3-O glucoside-7-O-rhamnoside, Luteolin 5-O-(6-O-malonylglucoside), Kaempferol 3-O-(6-O-acetylglucoside)-7-O-rhamnoside, Genkwanin 5-O-glucoside, Luteolin 5-O-(6-O-malonylglucoside), Kaempferol 3-O-(6-O-malonylglucoside), Kaempferol 3-O-rhamnoside, Quercetin, Genkwanin 4-O-glucoside and Hyperoside.
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Cėspedes CL, El-Hafidi M, Pavon N, Alarcon J. Antioxidant and cardioprotective activities of phenolic extracts from fruits of Chilean blackberry Aristotelia chilensis (Elaeocarpaceae), Maqui. Food Chem. 2008; 107: 820-829.
Kobiv Y, Kish R, Hleb R. Sempervivum marmoreum Griseb. (Crassulaceae) in the Ukrainian Carpathians: distribution, morphology, coenotic conditions, population parameters, and conservation. Ukrainian Botan J. 2007; 64: 23-29
Guarrera PM. Traditional phytotherapy in Central Italy (Marche, Abruzzo, and Latium). Fitoterapia. 2005; 76: 1-25.
Pieroni A, Dibra B, Grishaj G, Grishaj I, Macai G. Traditional phytotherapy of the Albanians of Lepushe, Northern Albanian Alps. Fitoterapia. 2005; 76: 379-399
Graham GJ, Quinn LM, Fabricant DS, Farnsworth NR. Plants used against cancer-an extension of the work of Jonathan Hartwell. J Ethnopharmacol. 2000; 73: 347-377.
Jäger AK, Guguin B, Adsersen A, Gudiksen L. Screening of plants used in Danish folk medicine to treat epilepsy and convulsions. J Ethnopharmacol. 2006; 105: 294-300.
Abram V, Donko M. Tentative Identification of Polyphenols in Sempervivum tectorum and Assessment of the Antimicrobial Activity of Sempervivum L., J Agr Food Chem. 1999; 47: 485-489.
Kery A, Blazovics A, Rozlosnik N, Feher J, Petri, G. Antioxidative properties of extracts from Sempervivum tectorum, Planta Med. 1992; 58: A661-A662.
Blázovics A, Lugasi T, Kemény K, Hagymási, Kéry Á. Membrane stabilising effects of natural polyphenols and flavonoids from Sempervivum tectorum on hepatic microsomal mixed-function oxidase system in hyperlipidemic rats. J Ethnopharmacol. 2000; 73: 479-485.
Blázovics A, González‐Cabello R, Barta I, Gergely P, Fehér J, Kéry Å, Petri, G. Effect of liver‐protecting Sempervivum tectorum extract on the immune reactivity of spleen cells in hyperlipidaemic rats. Phytother Res. 1994; 8: 33-37.
Blázovics A, Fehér J, Fehér E, Kéry, A, Petri, G. Liver protecting and lipid lowering effect of Sempervivum tectorum. Phytother Res. 1993; 7: 98-100.
Stojičević SS, Stanisavljević IT, Veličković DT, Veljković VB, Lazić ML. Comparative screening of the anti-oxidant and antimicrobial activities of Sempervivum marmoreum L. extracts obtained by various extraction techniques, J Serb Chem Soc. 2008; 73: 597-607.
Chemat F, Rombaut N, Sicaire AG, Meullemiestre A, Fabiano-Tixier AS, Abert-Vian M. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason Sonochem. 2017; 34: 540–560.
Suslick KS, Price GJ. Applications of ultrasound to materials chemistry. Annu Rev Mater Sci. 1999; 29: 295-326.
Vinatoru M. An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrason Sonochem. 2001; 8: 303-313.
Nikitenko SI, Chemat F, Ultrasound in Process Engineering, New Look at Old Problems, In: Poux М, Cognet P, Gourdon C, ed. Green Process Engineering From Concepts to Industrial Applications. CRC Press, 2015: 145–165.
Chemat F, Albert-Vian M, Cravotto G. Green Extraction of Natural Products: Concept and Principles. Int J Mol Sci. 2012; 13: 8615-8627.
Virot M, Tomao V, Le Bourvellec C, Renard CMCG, Chemat F. Towards the industrial production of antioxidants from food processing by-products with ultrasound-assisted extraction. Ultrason Sonochem.2010; 17: 1066–1074.
Khan KM, Albert-Vian M, Fabiano-Tixier A-S. Ultrasound-assisted extraction of polyphenols (flavonone glucosides) from orange (Citrus sinensis L.) peel. Food Chem. 2010; 119: 851-858.
Pico Y. Ultrasound-assisted extraction for food and environmental samples. Trends Anal Chem. 2013; 43: 84-99.
Rombaut N, Tixier A-S, Bily A, Chemat F. Green extraction process of natural products as tool for biorefinery. Biofuels, Bioprod Bioref. 2014; 8: 530-544.
Tabaraki R, Heidarizadi E, Benvidi A. Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) peel antioxidants by response surface methodology. Sep Pur Technol. 2012; 98: 16-23.
Pan Z, Qu W, Ma H, Atungulu GG, McHugh HT. Continuous and pulsed ultrasound-assisted extractions of antioxidants from pomegranate peel. Ultrason. Sonochem. 2012; 19: 365-372.
Smelcerovic A, Spiteller M, Zuehlke S. Comparison of Methods for the Exhaustive Extraction of Hypericins, Flavonoids, and Hyperforin from Hypericum perforatum L. J Agric Food Chem. 2006; 54: 2750-2753.
Saleh IA, Vinatoru M, Mason TJ, Abdel-Azim NS, Aboutabl EA, Hammouda FM. Ultrasonic-assisted extraction and conventional extraction of Silymarin from Silybum marianum seeds; a comparison. Res J Pharm Biol Chem Sci. 2015; 6: 709-717.
Wu J, Lin LD, Chau FT, Ultrasound-assisted extraction of ginseng saponins from ginseng roots and cultured ginseng cells. Ultrason Sonochem. 2001; 8:347-352.
Vinatoru M, Maricela T, Radu O, Filip PI, Lazurca D, Mason TJ. The use of ultrasound for the extraction of bioactive principles from plant materials. Ultrason Sonochem. 1997; 4: 135–139.
Singleton VL, Rossi JA. Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents, Amer J Viticult Enol. 1965; 16: 144-158.
Dias, ALB, Arroio Sergio, CS, Santos, P, Barbero GF, Rezende, CA, Martínez, J, Ultrasound-assisted extraction of bioactive compounds from dedo de moça pepper (Cap-sicum baccatum L.): Effects on the vegetable matrix and mathematical modeling, J Food Eng. 2017; 198: 36-44.
Jadhav D, Rekha BN, Gogate PR, Rathod VK. Extraction of vanillin from vanilla pods: a comparison study of conventional soxhlet and ultrasound assisted extraction. J Food Eng. 2009; 93: 421–426.
Boonkird S, Phisalaphong C, Phisalaphong M. Ultrasound-assisted extraction of capsaicinoids from Capsicum frutescens on a lab- and pilot-plant scale, Ultrason Sonochem. 2008; 15: 1075-1079
Dent M, Dragović-Uzelac V, Penić M, Brnčić M, Bosiljkov T,Levaj B. The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in Dalmatian wild sage (Salvia officinalis L.) extracts. Food Technol Biotechnol. 2013; 51: 84-91.
Choi CW, Kim SC, Hwang SS, Choi KB, Ahn JH, Lee MY., Parkand SH, Kim SK. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci. 2002; 163: 1161-1168.
Brand-Williams W, Cuvelier EM, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol. 1995; 28: 25–30.
Viet M, Beckert C, Höhne C, Bauer K, Geiger H. Interspecific and intraspecific variation of phenolics in the genus Equisetum subgenus Equisetum. Phytochem. 1995; 38: 881-891.
Barbero GF, Liazid A, Palma M, Barroso CG. Ultrasound-assisted extraction of capsaicinoids from peppers, Talanta 2008; 75: 1332–1337.
Zu G, Zhang R, Yang L, Ma C, Zu Y, Wang W, Zhao C. Ultrasound-assisted extraction of carnosic acid and rosmarinic acid using ionic liquid solution from Rosmarinus officinalis. Int J Mol Sci. 2012; 13: 11027-11043.
Petigny L, Périno-Issartier S, Wajsman J, Chemat F. Batch and continuous ultrasound assisted extraction of boldo leaves (Peumus boldus Mol.). Int J Mol Sci. 2013; 14: 5750-5764.
Wang H-J, Pan, M-C, Chang C-K, Chang S-W, Hsieh C-W. Optimization of ultrasonic-assisted extraction of cordycepin from Cordyceps militaris using orthogonal experimental design. Molecules 2014; 19: 20808-20820.
Esclape MD, García-Pérez JV, Mulet A, Cárcel JA. Ultrasound-Assisted Extraction of Natural Products, Food Eng Rev. 2011; 3: 108-120.
Sharmila G, Nikitha VS, Ilaiyarasi S, Dhivyaa K, Rajasekara V, Kumarb NM, Muthukumaran K, Muthukumaran C. Ultrasound assisted extraction of total phenolics from Cassia auriculata leaves and evaluation of its antioxidant activities. Ind Crops Prod. 2016; 84: 13-21.
Ghasemzadeh A, Jaafar HZE, Karimi E, Rahmat A. Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology. BMC Complement Altern Med. 2014; 14: 318-328.
Upadhyay R, Nachiappan G, Mishra HN. Ultrasound-assisted extraction of flavonoids and phenolic compounds from Ocimum tenuiflorum leaves. Food Sci Biotechnol. 2015; 24: 1951-1958.
Vajnhandl S, Marechal AML. Ultrasound in textile dyeing and the decolourization/ mineralization of textile dyes. Dyes Pigm. 2005; 65: 89–101.
Wu C, Liu X, Wei D, Fan J, Wang L. Photosonochemical degradation of Phenol in water. Water Research. 2001; 35: 3927-3933.
Paniwnyk L, Beaufoy E, Lorimer PJ, Mason TJ. The extraction of rutin from flower buds of Sophora japonica. Ultrason Sonochem. 2001; 8: 299-301.
Carciochi RA, Manrique GD, Dimitrov K. Optimization of antioxidant phenolic compounds extraction from quinoa (Chenopodium quinoa) seeds. J Food Sci Technol. 2015; 52: 4396–4404.
Biesaga M. Influence of extraction methods on stability of flavonoids. J Chromatogr A. 2011; 1218: 2505–2512.
Vuong QV, Goldsmith CD, Dang TT, Nguyen VT, Bhuyan DJ, Sadeqzadeh E, Scarlett CJ, Bowyer MC. Optimisation of Ultrasound-Assisted Extraction Conditions for Phenolic Content and Antioxidant Capacity from Euphorbia tirucalli Using Response Surface Methodology. Antioxidants 2014; 3: 604-617.
Portenlanger G. Heusinger H, The influence of frequency on the mechanical and radical effects for the ultrasonic degradation of dextranes. Ultrason Sonochem. 1997; 4: 127-130.
Alberti A, Szabolcs B, Lackoc E, Ribac P, Al-Khrasanic M, Kerya A. Characterization of phenolic compounds and antinociceptive activity of Sempervivum tectorum L. leaf juice. J Pharm Biomed Anal. 2012; 70: 143-150.
Alberti A, Blazics B, Kery A. Evaluation of Sempervium tectorum L., flavonoids by LC and LC-MS. Chromatographia. 2008; 68: 107-111.
Gumenyuk AL. Scutellarein 7-rutinoside from Sempervivum ruthenicum. Chem Nat Compd. 1975; 3: 428-429.
Gumenyuk LA, Phenolic carboxylic acids from Sempervivum ruthenicum. Chem Nat Compd. 1971; 7: 503-503
Stevens JF, Hart H, Elema ET, Bolck A. Flavonoid variation in Eurasian Sedum and Sempervivum. Phytochemistry 1996; 41: 503–512.