RATIONAL FEEDING STRATEGIES OF SUBSTRATE AND ENZYMES TO ENZYMATIC HYDROLYSIS BIOREACTORS
Scientific paper
DOI:
https://doi.org/10.2298/CICEQ201202030PKeywords:
Enzymatic hydrolysis, Fed-batch operation, Rational feeding strategies, Sugarcane straw, Unproductive lignin-enzyme bondsAbstract
Bioreactors operating in fed-batch mode improve the enzymatic hydrolysis productivity at high biomass loadings. The present work aimed to apply rational feeding strategies of substrates (pretreated sugarcane straw) and enzymes (CellicCtec2®) to achieve sugar titers at industrial levels. The instantaneous substrate concentration was kept constant at 5% (w/v) along the fed-batch. The enzyme dosage inside the bioreactor was adjusted so that the reaction rate was not less than a pre-defined value (a percentage of the initial reaction rate – rmin). When r reached values below rmin, enzyme pulses were applied to return the reaction rate to its initial value (r0). The optimized feeding policy indicated a reaction rate maintained at a minimum of 70% of r0, based on the trade-off between glucose productivity and enzyme saving. Initially, it was possible to process a 21% (w/v) solid load, achieving 160 g/L of glucose concentration and 80% of glucose yield. It was verified that non-productive enzyme adsorption was the main reason for some reduction of hydrolysis yield regarding the theoretical cellulose-to-glucose conversion. An increment of 30 g/L in the final glucose concentration was achieved when a lignin-blocking additive (soybean protein) was used in the enzymatic hydrolysis.
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Copyright (c) 2022 Bruna Pratto, Martha Suzana Rodrigues dos Santos-Rocha, Gustavo Gustavo, Inti Doraci Cavalcanti-Montaño, Carlos Alberto Suarez Galeano, Antonio José Gonçalves Cruz, Ruy de Sousa Júnior
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