RATIONAL FEEDING STRATEGIES OF SUBSTRATE AND ENZYMES TO ENZYMATIC HYDROLYSIS BIOREACTORS Scientific paper

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Bruna Pratto
Martha Suzana Rodrigues dos Santos-Rocha
Gustavo Batista
https://orcid.org/0000-0002-7023-9792
Inti Doraci Cavalcanti-Montaño
Carlos Alberto Galeano Suarez
Antonio José Gonçalves Cruz
Ruy de Sousa Júnior
https://orcid.org/0000-0003-4916-173X

Abstract

Bioreactors operating in fed-batch mode improve the enzymatic hydrolysis productivity at high biomass loadings. The present work aimed to apply rational feeding strategies of substrates (pretreated sugarcane straw) and enzymes (CellicCtec2®) to achieve sugar titers at industrial levels. The instantaneous substrate concentration was kept constant at 5% (w/v) along the fed-batch. The enzyme dosage inside the bioreactor was adjusted so that the reaction rate was not less than a pre-defined value (a percentage of the initial reaction rate – rmin). When r reached values below rmin, enzyme pulses were applied to return the reaction rate to its initial value (r0). The optimized feeding policy indicated a reaction rate maintained at a minimum of 70% of r0, based on the trade-off between glucose productivity and enzyme saving. Initially, it was possible to process a 21% (w/v) solid load, achieving 160 g/L of glucose concentration and 80% of glucose yield. It was verified that non-productive enzyme adsorption was the main reason for some reduction of hydrolysis yield regarding the theoretical cellulose-to-glucose conversion. An increment of 30 g/L in the final glucose concentration was achieved when a lignin-blocking additive (soybean protein) was used in the enzymatic hydrolysis.

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Pratto, B., Rodrigues dos Santos-Rocha, M. S., Batista, G., Cavalcanti-Montaño, I. D., Galeano Suarez, C. A., Gonçalves Cruz, A. J., & de Sousa Júnior, R. (2022). RATIONAL FEEDING STRATEGIES OF SUBSTRATE AND ENZYMES TO ENZYMATIC HYDROLYSIS BIOREACTORS: Scientific paper. Chemical Industry & Chemical Engineering Quarterly, 28(3), 191–201. https://doi.org/10.2298/CICEQ201202030P
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