USE OF AN INTERNAL LOOP AIRLIFT BIOREACTOR TO PRODUCE POLYHYDROXYALKANOATES BY Stenotrophomonas rhizophila

Original scientific paper

Authors

  • Berenice Clifton-García Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara. Olímpica, Guadalajara, Jal, Mexico
  • Juan Villafaña-Rojas Departamento de Biotecnológicas y Ambientales, Universidad Autónoma de Guadalajara, Zapopan, Jal., Mexico
  • Orfil González-Reynoso Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara. Olímpica, Guadalajara, Jal., Mexico
  • Jorge Ramon Robledo-Ortiz Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara. Guadalajara, México
  • Ricardo Manríquez-González Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara. Olímpica, Guadalajara, Jal, Mexico
  • Porfirio Gutiérrez-González Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara. Olímpica, Guadalajara, Jal.
  • Yolanda González-García Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara. Olímpica, Guadalajara, Jal, Mexico

DOI:

https://doi.org/10.2298/CICEQ230819014C

Keywords:

Airlift bioreactor, polyhydroxyalkanoates, molasses, Stenotrophomonas rhizophila

Abstract

Airlift-type bioreactors present advantages over conventional systems such as efficient mass transfer, simplicity of construction, and low energy consumption. Thus, they are a good alternative for the production of polyhydroxyalkanoates (PHAs) nevertheless, their use for that purpose has been barely studied. This work addresses the design, construction, and hydrodynamic characterization of a 2.4 L internal loop airlift bioreactor, evaluating the effect of the airflow, liquid volume, and disperser position, on the interfacial area and the mixing time. Then, it was used for the fed-batch production of PHB by Stenotrophomonas rhizophila from sugar cane molasses. It was found that the conditions to increase the interfacial area and minimize the mixing time were: airflow of 1.5 vvm, liquid volume of 2400 mL, and disperser position of 5 mm (distance between the air disperser and the drag tube). Under that configuration, the maximum biomass concentration, PHB production, and PHB accumulation achieved (54 h of culture) were 65.4 g/L, 39.9 g/L, and 60.2 % (g of PHB/100 g dry biomass), respectively. The polymer was poly-3-hydroxybutyrate, with a melting temperature of 170 °C, crystallinity of 56.4 %, and a Mw of 735 kDa.

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03.03.2025

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Vol. 31 No. 1 (2025)

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Copyright (c) 2025 Berenice Clifton-García, Juan Villafaña-Rojas, Orfil González-Reynoso, Jorge Ramon Robledo-Ortiz, Ricardo Manríquez-González, Porfirio Gutiérrez-González, Yolanda González-García

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How to Cite

USE OF AN INTERNAL LOOP AIRLIFT BIOREACTOR TO PRODUCE POLYHYDROXYALKANOATES BY Stenotrophomonas rhizophila: Original scientific paper. (2025). Chemical Industry & Chemical Engineering Quarterly, 31(1), 71-82. https://doi.org/10.2298/CICEQ230819014C

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