Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

Slavica M. Grujić, Ana M. Džamić, Violeta D. Mitić, Vesna Stankov-Jovanović, Petar D. Marin, Gordana S. Stojanović


Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS,FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin–Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloro­form (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 µmol Fe/mg. The best radical scavenger was methanol (1.04 µmol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxid­ant activity. The best reducing capacity was obtained with methanol extract of L. purpu­reum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.


Lamium purpureum, antioxidant activity, DPPH, ABTS, FRAP, TRP

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P.W. Ball, in: T.G. Tutin, V.H. Heywood, N.A. Burges, D.M. Moore, D.H. Valentine, S.M. Walters (Eds.), Flora Europaea, Vol. 3, Cambridge University Press, Cambridge, 1972.

R.F. Weiss, Weiss’s Herbal Medicine, Thieme, 2001.

N.G. Bisset, Herbal Drugs and Phytopharmaceuticals: A Handbook for Practice on a Scientific Basis. Scientific Publishers, Stuttgart, 1994, pp. 288–291.

E. Capecka, A. Mareczek, M. Leja, Antioxidant activity of fresh and dry herbs of some Lamiaceae species, Food Chem. 93 (2005) 223–226.

U. Ozgen, A. Mavi, Z. Terzi, A. Yildirim, M. Coskun, P.J. Hoghton, Antioxidant properties of some medicinal Lamiaceae (Labiatae) species, Pharm. Biol. 44 (2006) 107–112.

A. Wojdylo, J. Oszmianski, R. Czemerus, Antioxidant activity and phenolic compounds in 32 selected herbs, Food Chem. 105 (2007) 940–947.

D. Krishnaiah, R. Sarbatly, R. Nithyanandam, A review of the antioxidant potential of medicinal plant species, Food Bioprod. Process. 89 (2011) 217–233.

D. Prochazkova, I. Bousova, N. Wilhelmova, Antioxidant and prooxidant properties of flavonoids, Fitoterapia 82 (2011) 513–523.

C. Shuia, C. Xingguo, H. Zhide, Identification and deter-mination of ecdysone and phenylpropanoid glucoside and flavonoids in Lamium maculatum by capillary zone electrophoresis, Biomed. Chrom. 17 (2003) 477–482.

K. Alpieva, R. Taskova, Lj. Evstatieva, N. Handjieva, S. Popov, Benzoxazinoids and iridoids glucosides from four Lamium species, Phytochem., A 64 (2003) 1413–1417.

K. Alpieva, Lj. Evstatieva, N. Handijeva, S. Popov, Comparative analysis of the composition of flower volatiles from Lamium species and Lamiastrum galeobdolon Heist. ex Fabr., Z. Naturforsch., B 58 (2003) 779–782.

G. Flamini, P.L. Cioni, I. Moreli, Composition of the essential oils and in vivo emision of volatiles of four Lamium species from Italy: L. purpureum, L. hybridum, L. bifidum and L. amplexicaule, Food Chem. 91 (2005) 63–


K. Alpieva, R. Taskova, S. Jensen, N. Handjieva, Iridoid glucosides from Lamium album and Lamium maculatum (Lamiaceae), Biochem. Syst. Ecol. 34 (2006) 88–91.

T. Ersoz, D. Kaya, F.N, Yalcin, C. Kazaz, E. Palaska, C. Gotfredsen, S. Jensen, I. Calis, Iridoid glucoside from Lamium garganicum subsp. laevigatum, Turk. J. Chem. 31 (2007) 155–162.

F.N. Yalcin, D. Kaya, Ethnobotany, pharmacology and phytochemistry of the genus Lamium (Lamiaceae), J. Pharm. Sci. 31 (2006) 43–52.

K. Alpieva, T. Kokubin, R. Taskova, Lj. Evstatieva, N. Handjieva, LC-ESI-MS analysis of iridoid glucosides in Lamium species, Bioch. Syst. Ecol. 35 (2007) 17–22.

F.N. Yalcin, D. Kaya, I. Calis, T. Ersoz, E. Palaska, Determination of iridoid glucosides from four turkish Lamium species by HPLC-ESI/MS, Turk. J. Chem. 32 (2008) 457–467.

B. Halliwell, Antioxidant characterization: Methodology and mechanism, Biochem. Pharmacol. 49 (1995) 1341–1348.

A. Prakash, F. Rigelhof, E. Miller, Antioxidant activity. Medallion Laboratories, Anal. Progr. 19 (2001) 1–4.

F.N. Yalcin, D. Kaya, E. Kilic, M. Ozalp, T. Ersoz, I. Calis, Antimicrobial and free radical scavening activities of some Lamium species from Turkey, J. F. Pharm. 27 (2007) 11–22.

A. Matkowski, P. Tasrz, E. Szypuła, Antioxidant activity of herb extracts from five of L. purpureum medicinal plants from Lamiaceae, subfamily Lamioideae, J. Med. Pl. Res. 2 (2008) 321–330.

O. Yumrutas, S.D. Saygidegar, Determination of in vitro antioxidant activity of different extracts of Marrubium parviflorum Fish et Mey. and Lamium amplexicaule L. from South east of Turkey, J. Med. Pl. Res. 4 (2010) 2164–2172.

P. Trouillas, C.A. Calliste, D.P. Allais, A. Simon, A. Marfak, C. Delge, J.L. Duroux, Antioxidant, antiinflamatory and antiproliferative properties of sixteen water plant extracts used in the Limousin Countryside as herbal teas, Food Chem. 80 (2003) 399–407.

A. Matkowski, M. Piotrowska, Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae, Fitoterapia 77 (2006) 346–353.

J. Budzianowski, A. Budzianowska, Chromatographic and spectrophotometric analyses of the DPPH free radical scavenging activity of the fractionated extracts from Lamium album L., Lamium purpureum L., and Viscum album L., Herba Polonica 52 (2006) 52–57.

R. Paduch, M. Wojciak-Kosior, G. Matysik. Investigation of biological activity of Lamii albi flos extracts, J. Ethno-pharmacol. 110 (2007) 69–75.

E.K. Akkol, F.N. Yalcin, D. Kaya, I. Calis, E. Yesilada, T. Ersoz, In vivo anti inflamatory and nociceptive action of some Lamium species, J. Ethnopharmacol. 118 (2008) 166–172.

V.L. Singleton, J.A. Rossi. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Vitic. 16 (1965) 144–158.

Y.K. Park, M.H. Koo, M. Ikegaki, J.L. Contado, Comparison of the flavonoid aglycone contents of Apis mellifera propolis from various regions of Brazil, Arq. Biol. Tecnol. 40 (1997) 97–106.

M.S. Blois, Antioxidant determination by the use of stabile free radical, Nature 181 (1958) 1199–1200.

C.A. Rice-Evans, N.J. Miller, Total antioxidant status in plasma and body fluids, Methods Enzymol. 234 (1994) 279–293.

R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radical Bio. Med. 26 (1999) 1231–1237.

I.F.F. Benzie, J.J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay, Anal. Biochem. 239 (1996) 70–76.

M. Oyaizu, Studies on product of browning reaction prepared from glucoseamine, Jpn. J. Nutr. 44 (1986) 307–315.

C. Bubueanu, G. Campeanu, L. Pirvu, G. Bubueanu, Anti-oxidant activity of butanolic extracts of romanian native species – Lamium album and Lamium purpureum, Roman. Biotechnol. Lett. 18 (2013) 8855–8862.

B. Nickavar, M. Kamalinejad, H. Izapanah, In vitro free radical scavenging activity of five Salvia species, Pak. J. Pharm. Sci. 20 (2007) 291–294.

F. Sharififar, G. Dehghn-Nudeh, M. Mirtajaldini, Major flavonoids with antioxidant activity from Teucrium polium L., Food Chem. 112 (2009) 885–888.

L.L. Mensor, F.S. Menezes, G.G. Leitao, A.S. Reis, T.C. Santos, C.S. Coube, S.G. Leitao, Screening of brazilian plant extracts for antioxidant activity by the use of DPPH free radical method, Phytother. Res. 15 (2001) 127–130.

H.B. Li, C.C. Wong, K.W. Cheng, F. Chen, Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants, LWT Food Sci. Technol. 41 (2008) 385–390.

B. Kaurinovic, M. Popovic, S. Vlaisavljevic, S. Trivic, Anti-oxidant capacity of O. basilicum L. and Origanum vulgare L. extracts, Molecules 16 (2011) 7401–7414.

J. Kanner, E.N. Frankel, R. Grant, J.B. German, J.E. Kin-sella, Natural antioxidants in grapes and wines, J. Agric. Food Chem. 42 (1994) 64–69.

M. Hidalgo, C. Sanches Moreno, S. de Pascual Teresa, Flavonoid-flavonoid interaction and its effect on their antioxidant activity, Food Chem. 121 (2010).

M.S. Valyova, M.A. Dimitrova, Y.A. Ganeva, V.M. Kapchina-Toteva, Z.P. Jordanova. Evaluation of antioxidant and free radical scavenging potential of Lamium album L. growing in Bulgaria, J. Pharm. Res. 4 (2011) 945–947.

A.R. Gohari, H. Hajimehdipoor, S. Saeidnia, Y. Ajani, A. Hadjiakhoondi, Antioxidant activity of some medicinal species using FRAP assay, J. Med. Plant. 10 (2011) 54–60.

D. Mantle, F. Eddeb, A.T. Pickering, Comparison of rel-ative antioxidant activities of british medicinal plant species in vitro, J. Ethnopharmacol. 72 (2000) 47–51.

M. Chaalal, N. Touati, H. Louaileche, Extraction of phe-nolic compounds and in vitro antioxidant capacity of prickly peer seed, Acta Bot. Gall. 159 (2012) 467–475.

Y. Benchikh, H. Louaileche, Effects of extraction condition on the recovery of phenolic compounds and in vitro antioxidant activity of carob (Ceratonia siliqua L.) pulp, Acta Bot. Gall. 161 (2014) 175–181.

DOI: http://dx.doi.org/10.2298/HEMIND160518047G


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