Antimicrobial activity of Helichrysum plicatum DC.

Dubravka J. Bigović, Tatjana R. Stević, Teodora R. Janković, Nebojša B. Noveski, Dragoja S. Radanović, Dejan S. Pljevljakušić, Zorica R. Djurić


Dry flower heads of Helichrysum plicatum were characterized by HPLC-DAD and a detailed antimicrobial assay of its ethanol extract was performed. Identification of phenolic com­pounds indicated the presence of apigenin, naringenin and kaempferol as free aglycones, glycosides of apigenin, naringenin, quercetin and kaempferol as well as chlorogenic acid and chalcone derivate. Antimicrobial activity of the extract was evaluated against various bacteria and fungi as well as yeast Candida albicans using microdilution method. Gram-positive bacteria were more sensitive to the tested extract (MIC values were to 0.02 mg/mL) than Gram-negative bacteria (the greatest MIC was 0.055 mg/mL). Regarding pathogenic fungi, our tests demonstrated that fungi were more sensitive to the tested ext­ract than bacteria. The growth of the majority of the tested fungi was inhibited by concen­tration of 0.005 mg/mL. Moreover, the extract was significantly more active than com­mercial fungicide, fluconazole. The results of our tests indicate that the extract of H. plica­tum has significant antimicrobial activity and may find application in the pharmaceutical and food industry and organic agriculture.


Helichrysum plicatum; antimicrobial activity; naringenin; kaempferol; apigenin

Full Text:

PDF (308 kB)


A.R. Clapham, in: T.G. Tutin, V.H. Heywood, N.A.Burges, D.H., Valentine, S.M. Walters, & D.A. Webb (Eds.), 24. Helichrysum Miller, Flora europaea 4, Cambridge, 1976, pp. 128–131.

A.C.U. Lourens, A.M. Viljoen, F.R.van Heerden, South African Helichrysum species: A review of the traditional uses, biological activity and phytochemistry, J. Ethnopharmacol. 119 (2008) 630–652.

R.J. Bayer, I. Breitwieaser, J. Ward, C.F. Puttock, in: J.W. Kadereit and C. Jeffrey (Eds.), Tribe Gnaphalieae (Cass.) Lecoq & Juillet (1831), Families and genera of vascular plants, Vol. VIII: Asterales, Springer, Berlin, 2007, pp. 246–284.

V. Stevanović, Iščezli i krajnje ugroženi taksoni. Crvena knjiga Flore Srbije, Beograd, 1999, pp. 341.

C. Gusev, Helichrysum plicatum (Asteraceae) - a new species far the Bulgarian flora. Boccanea 5 (1997) 667–669.

N.J. Montvale, PDR for Herbal Medicines 3rd ed., Thomson PDR, Montvale, NJ, 2004, pp. 457–458.

M. Carini, G. Aldini, S. Furlanetto, R. Stefani, R. Facino, LC coupled to ion-trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas. J. Pharm. Biomed. Anal. 24 (2001) 517–526.

O.P. Prokopenko, V.N. Spiridonov, V.I. Litvinenko, V.T. Chornobai, G.V. Obolentseva, Y.I. Khadzhai, Z.I. Tatarko, Phenol compounds of Helichrysum and their biological activity, Farm. Zh. 27 (1972) 3–7.

A. Sala, M.C. Recio, G. Schinella, S. Mañez, R.M. Giner, M. Cerda-Nicolas, J.L. Rios, Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside, Eur. J. Pharmacol. 461 (2003) 53–61.

A. Sala, M.C. Recio, G. Schinella, S. Mañez, R.M. Giner, J.L. Rios, A new dual inhibitor of arachidonate metabolism isolated from Helichrysum italicum, Eur. J. Pharmacol. 460 (2003) 219–226.

A. Rosa, M. Deiana, A. Atzeri, G. Corona, A. Incani, M.P. Melis, G. Appendino, M.A. Dessi, Evaluation of the anti–oxidant and cytotoxic activity of arzanol, a prenylated α-pyrone-phloroglucinol etherodimer from Helichrysum italicum subsp. microphyllum, Chem.-Biol. Interact. 165 (2007) 117–126.

S. Kulevanova, M. Stefova, T. Stafilov, HPLC identification and determination of flavone aglycones in Helichrysum plicatum DC. (Asteraceae), Pharmazie 55 (2000) 391–392.

D. Bigović, K. Šavikin, T. Janković, N. Menković, G. Zdunić, T. Stanojković, Z. Djurić, Antiradical and cytotoxic activity of different Helichrysum plicatum flower extracts, Nat. Prod. Commun. 6 (2011) 819–822.

V.V. Smirnov, N.E. Preobrazhenskaya, I.D. Kalashnikov Antibacterial properties of Helichrysum plicatum DC., Mikrobiol. Zh. 44 (1982) 71–72.

B. Tepe, M. Sokmen, H. Askin Akpulat, A. Sokmen, In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey, Food Chem. 90 (2005) 685–689.

M. Aslan, D.D. Orhan, N. Orhan, E. Sezik, E. Yesilada, In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats, J. Ethnopharmacol. 109 (2007) 54–59.

D. Bigović, S. Branković, D. Kitić, M. Radenković, T. Jan-ković, K. Šavikin, S. Živanović, Relaxant effect of the ethanol extract of Helichrysum plicatum (Asteraceae) on isolated rat ileum contractions, Molecules 15 (2010) 3391–3401.

O. Turgay, E. Carkirogly, S. Karaman, Antibacterial Activities of Helichrysum plicatum Subsp . plicatum Extracts, Journal of Medicinal Sciences, 1 (2001) 176–178.

H. Hanel, W. Reaether, A more sophisticated method of determining the fungicidal effect of water-insoluble preparations with a cell harvester, using miconazole as an example, Mycoses 31 (1988) 148–154.

R. Daouk, H. Dagher, J. Sattout, Antifungal activity of the essential oil of Origanum syriacum L., J. Food Protect. 58 (1995) 1147–1149.

T. Stević, S. Pavlović, S. Stanković, K. Šavikin, Pathogenic microorganisms of medicinal herbal drugs, Arch. Biol. Sci. 64 (2012) 49–58.

S. Albayarak, O. Sagdic, A. Aksoy, E. Hamzaoglu, Antimicrobial and antioxidant activities of Helichrysum species from the Mediterranean region of Turkey, Asian J. Chem. 20 (2008) 3143–3152.

S. Albayarak, A. Aksoy, O. Sagdic, E. Hamzaoglu, Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae), Food Chem. 119 (2010) 114–122.

A. Stanojković, J. Petrović, Lj. Čomić, S. Ćurčić, Antibacterial activity of the water, ethanol and ethyl acetate extracts of Helichrysum arenarum (L.), Lek. Sirovine 23 (2003) 37–41.

A. Basile, S. Giordano, A. Lopez-Saez, R. Cobianchi, Antibacterial activity of pure flavonoids isolated from mosses, Phytochemistry 52 (1999) 1479–1482.

A. Saravanakumar, K. Venteshwaran, J. Vanitha, M. Ganesh, M. Vasudevan, T. Sivakumar, Evaluation of antibacterial activity, phenol and flavonoid contents of Thespesia populnea flower extracts, Pak. J. Pharm. Sci. 22 (2009) 282–286.

T.P. Cushnie, A.J. Lamb, Antimicrobial activity of flavonoids, Int. J. Antimic. Agents 26 (2005) 343–356.

C. Andrade, J. Carvalho, M. Cunico, A. Lordello, C. Higaskino, S. Almeid, J. Dias, V. Kerber, M. Miguel, O. Miguel, Antioxidant and antibacterial activity of extracts, fractions and isolated substances from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don, Braz. J. Pharm. Sci. 46 (2010) 715–721.

G. Mandalari, R.N. Bennett, G. Bisignano, D. Trombetta, A. Saija, C.B. Faulds, M.J. Gasson, A. Narbad, Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry, J. Appl. Microbiol. 103 (2007) 2056–2064.

O.K. Mirzoeva, R.N. Grishanin, P.C. Calder, Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria, Microbiol. Res. 152 (1997) 239–246.

K.A. Ohemeng, C.F. Schwender, K.P. Fu, J.F. Barrett, DNA gyrase inhibitory and antibacterial activity of some flavones (1), Bioorg. Med. Chem. Lett. 3 (1993) 225–230.

K. Pal, B. McSpadden Gardener, Biological Control of Plant Pathogens, The Plant Health Instructor 10 (2006) 1117–1142.

M.Abdel-Kader, N. El-Mougy, S. Lashin, Essential oils and Trichoderma harzianum as an integrated control measure against faba bean root pathogens, J. Plant Prot. Res. 51 (2011) 306–313.

E. Survilienė, A. Valiuškaitė, V. Snieškienė, A. Stankevičienė, Effect of essential oils on fungi isolated from apples and vegetables, Sodinikysté ir Daržininkysté 28 (2009) 227–234.



  • There are currently no refbacks.

Copyright (c) 2017 HEMIJSKA INDUSTRIJA

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.