Enhanced lipid extraction from microalgae in biodiesel production

Myung-Gyun Kim, Hyun-Wook Hwang, Antony Mutua Nzioka, Young-Ju Kim


In order to secure more effective lipid extraction method, this research investigated new lipid extraction method using laser with absorbent and sought its optimum operation control. In addition, this study compared lipid extraction efficiency and FAME conversion rate between laser extraction method at optimum condition and existing extraction method. Results from experiments for optimizing lipid extraction method using laser showed that the maximum extraction efficiency (81.8%) was attained when using laser with an output capacity of 75Wh/L. Extraction efficiency increased up to 90.8% when mic­rowave treatment as pretreatment process was conducted. Addition of absorbents during lipid extraction process with laser showed higher extraction efficiency than laser and chemical method. It was also found that laser extraction method with absorbent had higher total fatty acid content (853.7 mg/g oil) in extracted lipid than chemical extraction method (825.4 mg/g oil). Furthermore, it had the highest FAME conversion rate (94.2%).


biodiesel, microalgae, laser, lipid extraction

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H. E. Hoydonckx, D. E. De Vos, S. A. Chavan, P.A. Jacobs, Esterification and transesterification of renewable chemicals, Top. Catal. 27 (2004) 83–96.

Y. Chisti, Biodiesel from microalgae, Biotechnol. Adv. 25 (2007) 294–306.

T.P. Durrett, C. Benning, J. Ohlrogge, Plant triacylglycerols as feedstock for the production of biofuels, Plant J. 54 (2008) 593–607.

S. Amin, Review on biofuel oil and gas production processes from micro algae, Energ. Convers. Manage. 50 (2009) 1834–1840.

J.Y. Lee, C. Yoo, S.Y. Jun, C.Y. Ahn, H.M. Oh, Comparison of several methods for effective lipid extraction from microalgae, Bioresour. Technol. 101 (2007) S75–S77.

Y. Jung, Comparative analysis of lipid extraction from energy microalgae, Scenedesmus obliquus, Master’s Theses, Kyungpook National University, Daegu, 2012 (In Korean).

Q. Hu, M. Sommerfeld, E. Jarvis, M. Ghirardi, M. Posewitz, M. Seibert, A. Darzins, Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances, Plant J. 54 (2008) 621–639.

P.Y. Choi, Lipid extraction and and pretreatment esterification from microalgae, Master’s Theses, Korea University, Seoul, 2012 (In Korean).

C.L. Tautorus, Low possible causes for decreased stability of canola oil processed from green seed, J. Am. Oil Chem. Soc. 71 (1994) 1123–1128.

T. Issariyakul, A.K. Dalai, Biodiesel production from greenseed canola oil, Energy Fuels 24 (2010) 4652–4658.

M.J. Griffiths, S.T.L. Harrison, Lipid productivity as a key characteristic for choosing algal species for biodiesel production, J. Appl. Phycol. 21 (2009) 493–507.

E.G. Bligh, W.J. Dyer, A rapid method for total lipid extraction and purification, Can. J. Biochem. Physiol. 37 (1959) 911–917.

P. Prabakaran, A.D. Ravindran, A comparative study on effective cell disruption methods for lipid extraction from microalgae, Lett. Appl. Microbiol. 53 (2011) 150–154.

Y.H. Seo, M. Sung, Y.K. Oh, J.I. Han, Lipid extracvtion and esterification for microalgae-based biodiesel production using pyrite (FeS2), Bioresour. Technol. 191 (2015) 420–425.

A. Ranjan, V.S. Moholkar, Mechanistic assessment of microalgal lipid extraction, Ind. Eng. Chem. Res. 49 (2010) 2979–2985.

American Oil Chemists’ Society, AOCS Officical Method cd 3d-63: Acid Value. Official Method and Recommended practices of the AOCS, AOCS, Champaign, IL, 2009.

N.Y. Kim, Extraction, purification and optimization of the lipid for biodiesel from Scenedesmus sp., Master’s Thesis, Kangwon National University, Kangwon, 2010 (In Korean).

J.Y. Lee, Comparison of several methods for effective lipid extraction from microalgae, Bioresour. Technol. 101 (2010) S75–S77.

DOI: http://dx.doi.org/10.2298/HEMIND160327027K


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